Increasing protein charge state when using laser electrospray mass spectrometry

Journal of the American Society for Mass Spectrometry
Santosh KarkiRobert J Levis

Abstract

Femtosecond (fs) laser vaporization is used to transfer cytochrome c, myoglobin, lysozyme, and ubiquitin from the condensed phase into an electrospray (ES) plume consisting of a mixture of a supercharging reagent, m-nitrobenzyl alcohol (m-NBA), and trifluoroacetic acid (TFA), acetic acid (AA), or formic acid (FA). Interaction of acid-sensitive proteins like cytochrome c and myoglobin with the highly charged ES droplets resulted in a shift to higher charge states in comparison with acid-stable proteins like lysozyme and ubiquitin. Laser electrospray mass spectrometry (LEMS) measurements showed an increase in both the average charge states (Zavg) and the charge state with maximum intensity (Zmode) for acid-sensitive proteins compared with conventional electrospray ionization mass spectrometry (ESI-MS) under equivalent solvent conditions. A marked increase in ion abundance of higher charge states was observed for LEMS in comparison with conventional electrospray for cytochrome c (ranging from 19+ to 21+ versus 13+ to 16+) and myoglobin (ranging from 19+ to 26+ versus 18+ to 21+) using an ES solution containing m-NBA and TFA. LEMS measurements as a function of electrospray flow rate yielded increasing charge states with decreasing ...Continue Reading

References

Apr 5, 1987·Journal of Molecular Biology·S Vijay-KumarW J Cook
Aug 1, 1996·Journal of Mass Spectrometry : JMS·E R Williams
Dec 3, 1998·Journal of the American Society for Mass Spectrometry·L Konermann, D J Douglas
Feb 2, 1999·Journal of the American Society for Mass Spectrometry·T A FliggeM Przybylski
Nov 10, 2000·Journal of the American Society for Mass Spectrometry·A T IavaroneE R Williams
Apr 26, 2001·Analytical Chemistry·A T IavaroneE R Williams
May 15, 2003·Journal of the American Society for Mass Spectrometry·Andrea SchmidtThomas Dülcks
Jul 3, 2004·Journal of Chromatography. a·Jonathan G ShackmanRobert T Kennedy
Jul 14, 2007·Journal of the American Society for Mass Spectrometry·Jason S PageRichard D Smith
Aug 4, 2007·Molecular & Cellular Proteomics : MCP·David M GoodJoshua J Coon
Jan 30, 2008·The Analyst·Yohannes H RezenomKermit K Murray
Feb 16, 2008·Journal of the American Society for Mass Spectrometry·David TouboulRenato Zenobi
Jul 29, 2008·Journal of the American Society for Mass Spectrometry·Jason S SampsonDavid C Muddiman
Aug 8, 2008·Mass Spectrometry Reviews·Richard H PerryRobert J Noll
Dec 23, 2008·Journal of the American Society for Mass Spectrometry·Shirley H LomeliJoseph A Loo
Aug 18, 2009·Journal of the American Society for Mass Spectrometry·Harry J Sterling, Evan R Williams
Oct 27, 2009·Journal of the American Society for Mass Spectrometry·Shirley H LomeliJoseph A Loo
Apr 15, 2010·Rapid Communications in Mass Spectrometry : RCM·Jia LiuHai Luo
May 21, 2010·Rapid Communications in Mass Spectrometry : RCM·John J BradyRobert J Levis
Aug 18, 2010·Analytical Chemistry·Anastasia KharlamovaScott A McLuckey
Sep 30, 2010·Physical Chemistry Chemical Physics : PCCP·Christopher J HoganJuan Fernandez de la Mora
Mar 15, 2011·Physical Chemistry Chemical Physics : PCCP·Harry J SterlingEvan R Williams
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·John J BradyRobert J Levis
Jul 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·John J BradyRobert J Levis
Aug 2, 2011·Analytical Chemistry·Lorenzo TestaRita Grandori
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Harry J SterlingEvan R Williams
Oct 15, 2011·Journal of the American Society for Mass Spectrometry·Xiaohua Liu, Richard B Cole
May 9, 2012·Journal of the American Society for Mass Spectrometry·Zoe Hall, Carol V Robinson
Sep 6, 2012·Journal of the American Society for Mass Spectrometry·Tawnya G Flick, Evan R Williams
Feb 5, 2013·Journal of Mass Spectrometry : JMS·Shibdas Banerjee
Mar 5, 2013·Analytical Chemistry·Paul M FlaniganRobert J Levis
Nov 1, 1995·Journal of the American Society for Mass Spectrometry·P D SchnierE R Williams
Jul 1, 1993·Journal of the American Society for Mass Spectrometry·J B Fenn
Jan 28, 2014·Analytical Chemistry·Edward T ChainaniAlexander Scheeline

❮ Previous
Next ❯

Citations

Jan 8, 2017·Journal of the American Society for Mass Spectrometry·Santosh KarkiRobert J Levis
Mar 10, 2018·Journal of the American Society for Mass Spectrometry·Santosh KarkiRobert J Levis
Apr 15, 2018·Journal of the American Society for Mass Spectrometry·Jieutonne J ArcherRobert J Levis
Mar 17, 2017·Journal of the American Society for Mass Spectrometry·Habiballah SistaniRobert J Levis
Sep 11, 2019·Journal of the American Society for Mass Spectrometry·Anqi Tu, David C Muddiman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.