Increasing SARS-CoV-2 RT-qPCR testing capacity by sample pooling.

International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
J. Alcoba-FlorezCarlos Flores

Abstract

Limited testing capacity has characterized the ongoing coronavirus disease 2019 (COVID-19) pandemic in Spain, hampering timely control of outbreaks and opportunities to reduce the escalation of community transmission. This study investigated the potential to use sample pooling, followed by one-step retrotranscription and real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) to increase testing capacity for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Various pool sizes (five, 10 and 15 samples) were evaluated prior to RNA extraction followed by standard RT-qPCR for the diagnosis of COVID-19. The pool size achieving reproducible results with individual sample testing was subsequently used to assess nasopharyngeal samples in a tertiary hospital in August 2020. A pool size of five samples had higher sensitivity compared with pool sizes of 10 and 15 samples, showing a mean cycle threshold (Ct) shift of 3.5 [standard deviation (SD) 2.2] between the pooled test and positive samples in the pool. Next, a pool size of five was used to test a total of 895 pools (4475 prospective samples) using two different RT-qPCR kits. The Real Accurate Quadruplex corona-plus PCR Kit (PathoFinder) reported th...Continue Reading

References

Jun 4, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Julia Alcoba-FlorezCarlos Flores
Aug 4, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Julia Alcoba-FlorezCarlos Flores
Sep 1, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Francesca RegenJulian Hellmann-Regen
Sep 13, 2020·Science Advances·Noam ShentalTomer Hertz
Sep 14, 2020·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sanchita DasKaren M Frank
Oct 1, 2020·The New England Journal of Medicine·Michael J MinaDaniel B Larremore
Oct 17, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Fabiana VolpatoAfonso Luís Barth

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Citations

Mar 7, 2021·International Journal of Molecular Sciences·Stephen BustinTania Nolan
Apr 21, 2021·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Nadja GrobePeter Kotanko
Jul 1, 2021·Academic Pathology·Balaji BalasubramaniSrikar Chamala
Jun 28, 2021·Diagnostic Microbiology and Infectious Disease·Evangeline Ann DanielLuke Elizabeth Hanna
Jul 19, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yugan He, Yigang Tong
Jul 31, 2021·Open Forum Infectious Diseases·Stephen A RawlingsAntoine Chaillon
Aug 8, 2021·Diagnostic Microbiology and Infectious Disease·Marion MigueresJacques Izopet
Nov 11, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Naghib BogereIrene Andia-Biraro

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Methods Mentioned

BETA
PCR

Software Mentioned

R
PathoFinder
MedCalc

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