Increasing storage stability of freeze-dried plasma using trehalose

PloS One
Raffaele BrognaWillem F Wolkers

Abstract

Preservation of blood plasma in the dried state would facilitate long-term storage and transport at ambient temperatures, without the need of to use liquid nitrogen tanks or freezers. The aim of this study was to investigate the feasibility of dry preservation of human plasma, using sugars as lyoprotectants, and evaluate macromolecular stability of plasma components during storage. Blood plasma from healthy donors was freeze dried using 0-10% glucose, sucrose, or trehalose, and stored at various temperatures. Differential scanning calorimetry was used to measure the glass transition temperatures of freeze-dried samples. Protein aggregation, the overall protein secondary structure, and oxidative damage were studied under different storage conditions. Differential scanning calorimetry measurements showed that plasma freeze-dried with glucose, sucrose and trehalose have glass transition temperatures of respectively 72±3.4°C, 46±11°C, 15±2.4°C. It was found that sugars diminish freeze-drying induced protein aggregation in a dose-dependent manner, and that a 10% (w/v) sugar concentration almost entirely prevents protein aggregation. Protein aggregation after rehydration coincided with relatively high contents of β-sheet structures i...Continue Reading

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Citations

Sep 24, 2020·International Journal of Molecular Sciences·Isabel AndiaNicola Maffulli
Jul 17, 2021·Transfusion·Henry T PengAndrew Beckett
Jul 15, 2021·Microbiology Spectrum·Omar Anwar ElkadiMohammed A Ramadan
Aug 6, 2021·The Journal of Physical Chemistry Letters·Gil I OlgenblumDaniel Harries

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Methods Mentioned

BETA
infrared spectroscopy
light scattering
enzyme linked immunosorbent assay
PCA
ELISA

Software Mentioned

Omnic
Proteus

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