Increasing sulphite formation in Saccharomyces cerevisiae by overexpression of MET14 and SSU1

Yeast
U E Donalies, U Stahl

Abstract

Saccharomyces cerevisiae produces sulphite as an intermediate product during the assimilatory reduction of sulphate to sulphide. Three genes, MET3, MET14 and MET16, are essential for this reduction. We investigated the level of transcription of these genes in strains of S. cerevisiae with high, medium and low sulphite formation. The level of MET14- and MET16-mRNA varied with sulphite production, whereas the level of MET3-mRNA was very weak in almost all strains. We also analysed the effect of overexpression of MET14 and MET16 on sulphite formation. Two strains with low sulphite production were transformed with high-copy plasmids containing either or both MET14 and MET16. The overexpression of these two genes leads to a two- to three-fold sulphite formation. In addition, inactivation of MET10, encoding a subunit of the sulphite reductase, also leads to a distinct increase in sulphite formation; however, the cells became methionine auxotroph. The overexpression of SSU1, a gene encoding a putative sulphite pump, yields a slight increase in sulphite accumulation, whereas overexpression of SSU1, together with MET14, increases sulphite formation up to 10-fold. Furthermore, sulphite formation strongly depends on growth conditions, e.g...Continue Reading

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Citations

Feb 19, 2013·Eukaryotic Cell·Florian HennickePeter Staib
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·Elke Nevoigt
Mar 13, 2014·Applied Microbiology and Biotechnology·Julia C UsbeckRudi F Vogel
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Aug 28, 2020·Food Research International·Thomas OchandoVincent Farines

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