Increasing the carbon flux toward synthesis of short-chain-length--medium-chain-length polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae through modification of the beta-oxidation cycle

Applied and Environmental Microbiology
Valeria Cora De OliveiraY Poirier

Abstract

Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by using a mutant of the beta-oxidation-associated multifunctional enzyme with low dehydrogenase activity toward R-3-hydroxybutyryl coenzyme A.

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Nov 10, 1998·Trends in Biotechnology·A Steinbüchel, B Füchtenbusch
Apr 21, 1999·Current Opinion in Biotechnology·Y Poirier
Dec 10, 1999·Biotechnology Progress·J J HahnF Srienc
Feb 24, 2001·Advances in Biochemical Engineering/biotechnology·Y Poirier
Mar 12, 2002·FEMS Microbiology Letters·Yves PoirierJean MacDonald-Comber Petétot

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Citations

Jan 5, 2006·Applied and Environmental Microbiology·Bo ZhangFriedrich Srienc
Sep 13, 2015·Applied Microbiology and Biotechnology·Seiichi TaguchiHiromi Matsusaki
Apr 5, 2017·Organic & Biomolecular Chemistry·M B QuinC Schmidt-Dannert
Mar 15, 2005·Yeast
Jul 9, 2020·Synthetic and Systems Biotechnology·Xuan CaoYongjin J Zhou

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