Increasing the net charge and decreasing the hydrophobicity of bovine carbonic anhydrase decreases the rate of denaturation with sodium dodecyl sulfate.

Biophysical Journal
Katherine L GudiksenG M Whitesides

Abstract

This study compares the rate of denaturation with sodium dodecyl sulfate (SDS) of the individual rungs of protein charge ladders generated by acylation of the lysine epsilon-NH3+ groups of bovine carbonic anhydrase II (BCA). Each acylation decreases the number of positively charged groups, increases the net negative charge, and increases the hydrophobic surface area of BCA. This study reports the kinetics of denaturation in solutions containing SDS of the protein charge ladders generated with acetic and hexanoic anhydrides; plotting these rates of denaturation as a function of the number of modifications yields a U-shaped curve. The proteins with an intermediate number of modifications are the most stable to denaturation by SDS. There are four competing interactions-two resulting from the change in electrostatics and two resulting from the change in exposed hydrophobic surface area-that determine how a modification affects the stability of a rung of a charge ladder of BCA to denaturation with SDS. A model based on assumptions about how these interactions affect the folded and transition states has been developed and fits the experimental results. Modeling indicates that for each additional acylation, the magnitude of the change...Continue Reading

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Citations

Jun 1, 2013·Analytical and Bioanalytical Chemistry·Gustavo de Souza PessôaMarco Aurélio Zezzi Arruda
Oct 4, 2011·The Protein Journal·Srebrenka RobicShannon R McCartha
Oct 26, 2012·Journal of the American Chemical Society·Bryan F ShawGeorge M Whitesides
Nov 28, 2008·Journal of the American Chemical Society·Grégory F SchneiderGeorge M Whitesides
Aug 13, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Andrew LeeGeorge M Whitesides
Jul 5, 2008·Journal of Theoretical Biology·M R HousaindokhtM Bahrololoom
May 3, 2008·Protein Science : a Publication of the Protein Society·Bryan F ShawGeorge M Whitesides
Jun 14, 2012·Protein Science : a Publication of the Protein Society·Yunhua ShiBryan F Shaw
Jul 12, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Jacob L RogowskiFrank X Gu
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Jul 19, 2006·Chembiochem : a European Journal of Chemical Biology·Irina GitlinGeorge M Whitesides
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Dec 4, 2010·Journal of Agricultural and Food Chemistry·Ke ShiQingrong Huang
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