Increasing the sensitivity of an LC-MS method for screening material extracts for organic extractables via mobile phase optimization

Journal of Chromatographic Science
Yousheng Hua, Dennis Jenke

Abstract

Organic extractables (substances extracted from materials used in pharmaceutical packaging) are discovered, identified, and quantified via screening of extracts with analytical methods including liquid chromatography with mass spectrometric detection (LC-MS). Because extractables include a large number of diverse compounds that are typically present in plastic extracts at low levels, the LC-MS methods must be broad scope and sensitive. To accomplish these objectives, screening studies typically couple gradient reversed-phase separations with electrospray MS detection (both positive and negative ion modes). While such methods are generally applicable for a number of extractables, they are not optimal for some commonly encountered extractables due to either poor chromatographic performance (e.g., peak tailing) or poor MS response. Modifications to mobile phase composition (e.g., pH adjustment) were examined to improve the performance of an LC-MS screening method. The use of 0.1% acetic acid with 1 mM ammonium acetate (pH 3.6) as the aqueous portion of the mobile phase provided favorable sensitivities for a number of extractables both in positive and negative ion modes. In positive ion mode, the acidic mobile phase improved respon...Continue Reading

References

Feb 16, 2002·The Journal of Organic Chemistry·Akihiko Tsuda, Takumi Oshima
Mar 28, 2006·International Journal of Pharmaceutics·Dennis R JenkeRukhsana Lalani
Mar 8, 2008·Journal of the American Society for Mass Spectrometry·Brandie M EhrmannNadja B Cech
Jun 25, 2009·Mass Spectrometry Reviews·Paul Kebarle, Udo H Verkerk

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Citations

Jan 16, 2016·Analytical Chemistry·Michael WoldegebrielGabriel Vivó-Truyols
Feb 5, 2015·Mass Spectrometry Reviews·Zachary S BreitbachDaniel W Armstrong
Sep 21, 2016·Journal of the American Society for Mass Spectrometry·Babak BasiriMichael G Bartlett
Dec 15, 2016·Journal of the American Society for Mass Spectrometry·Jaanus LiigandAnneli Kruve
Mar 17, 2017·Journal of the American Society for Mass Spectrometry·Anneli Kruve, Karl Kaupmees
Jan 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vlad SerafimMihai Dinu Niculescu
Feb 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xin WangXiao-Ya Shang
Jul 1, 2016·The Journal of Applied Laboratory Medicine·Xin YiKiang-Teck J Yeo

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