Independent action between DvSnf7 RNA and Cry3Bb1 protein in southern corn rootworm, Diabrotica undecimpunctata howardi and Colorado potato beetle, Leptinotarsa decemlineata

PloS One
Steven L LevineJoshua P Uffman

Abstract

In recent years, corn rootworm (CRW)-resistant maize events producing two or more CRW-active Bt proteins have been commercialized to enhance efficacy against the target pest(s) by providing multiple modes of action (MoA). The maize hybrid MON 87411 has been developed that produces the CRW-active Cry3Bb1 Bt protein (hereafter Cry3Bb1) and expresses a RNAi-mediated MoA that also targets CRW. As part of an environmental risk assessment for MON 87411, the potential for an interaction between the CRW-active DvSnf7 RNA (hereafter DvSnf7) and Cry3Bb1 was assessed in 12-day diet incorporation bioassays with the southern corn rootworm (SCR, Diabrotica undecimpunctata howardi). The potential for an interaction between DvSnf7 and Cry3Bb1 was evaluated with two established experimental approaches. The first approach evaluated each substance alone and in combination over three different response levels. For all three response levels, observed responses were shown to be additive and not significantly different from predicted responses under the assumption of independent action. The second approach evaluated the potential for a fixed sub-lethal concentration of Cry3Bb1 to decrease the median lethal concentration (LC50) of DvSnf7 and vice-vers...Continue Reading

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Citations

May 27, 2015·Environmental Toxicology and Chemistry·Jianguo TanMichael H Beevers
Feb 16, 2017·Plant Biotechnology Journal·Mi NiZhen Zhu
May 25, 2016·Pest Management Science·Elane FishilevichBlair D Siegfried
Aug 17, 2019·Pest Management Science·Nitish KunteLuz-Adriana Avila
Mar 10, 2017·Frontiers in Plant Science·Huipeng PanXuguo Zhou
Feb 8, 2019·Pest Management Science·Simone HallerJörg Romeis
Jun 26, 2020·Frontiers in Plant Science·Jörg Romeis, Franco Widmer
Mar 22, 2021·Journal of Invertebrate Pathology·Steven L Levine

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Methods Mentioned

BETA
transgenic

Software Mentioned

GraphPad Prism
SAS

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