Independent effects of dietary fat and sucrose content on chondrocyte metabolism and osteoarthritis pathology in mice

Disease Models & Mechanisms
Elise L DonovanTimothy M Griffin

Abstract

Obesity is one of the most significant risk factors for knee osteoarthritis. However, therapeutic strategies to prevent or treat obesity-associated osteoarthritis are limited because of uncertainty about the etiology of disease, particularly with regard to metabolic factors. High-fat-diet-induced obese mice have become a widely used model for testing hypotheses about how obesity increases the risk of osteoarthritis, but progress has been limited by variation in disease severity, with some reports concluding that dietary treatment alone is insufficient to induce osteoarthritis in mice. We hypothesized that increased sucrose content of typical low-fat control diets contributes to osteoarthritis pathology and thus alters outcomes when evaluating the effects of a high-fat diet. We tested this hypothesis in male C57BL/6J mice by comparing the effects of purified diets that independently varied sucrose or fat content from 6 to 26 weeks of age. Outcomes included osteoarthritis pathology, serum metabolites, and cartilage gene and protein changes associated with cellular metabolism and stress-response pathways. We found that the relative content of sucrose versus cornstarch in low-fat iso-caloric purified diets caused substantial differ...Continue Reading

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Citations

Oct 1, 2020·Biomolecules·Iurii KobozievNaima Moustaid-Moussa
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Albert BatushanskyKenneth M Humphries
Jan 7, 2021·Tissue Engineering. Part C, Methods·Meagan J MakarczykHang Lin
Mar 24, 2021·Arthritis Research & Therapy·Geoffrey J KerrCheryle A Séguin
Aug 10, 2021·World Journal of Stem Cells·Li-Fang GuYan-Zhou Yang

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Methods Mentioned

BETA
X-ray
blood collection
dissection
reverse transcription PCR
PCR
electrophoresis

Software Mentioned

SkyLine
Cytoscape
igraph package
Prism

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