PMID: 2115870Aug 1, 1990Paper

Independent genes for two threonyl-tRNA synthetases in Bacillus subtilis

Journal of Bacteriology
Harald PutzerMarianne Grunberg-Manago

Abstract

With the exception of Escherichia coli lysyl-tRNA synthetase, the genes coding for the different aminoacyl-tRNA synthetases in procaryotes are always unique. Here we report on the occurrence and cloning of two genes (thrSv and thrS2), both encoding functional threonyl-tRNA synthetase in Bacillus subtilis. The two proteins share only 51.5% identical residues, which makes them almost as distinct from each other as each is from E. coli threonyl-tRNA synthetase (42 and 47%). Both proteins complement an E. coli thrS mutant and effectively charge E. coli threonyl tRNA in vitro. Their genes have been mapped to 250 degrees (thrSv) and 344 degrees (thrS2) on the B. subtilis chromosome. The regulatory regions of both genes are quite complex and show structural similarities. During vegetative growth, only the thrSv gene is expressed.

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Citations

Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·H D Becker, D Kern
Jul 1, 1997·Journal of Molecular Evolution·J R BrownW F Doolittle
Jun 15, 2011·Nature Reviews. Microbiology·Cheryl P Andam, J Peter Gogarten
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Ana Gutiérrez-PreciadoEnrique Merino
Apr 1, 2021·Nucleic Acids Research·Soumaya LaalamiHarald Putzer
Jan 23, 1999·Current Opinion in Genetics & Development·R F Doolittle, J Handy
Jan 1, 1994·Journal of Bacteriology·N GendronM Grunberg-Manago
Nov 1, 1996·Journal of Clinical Microbiology·W van LeeuwenA van Belkum

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