Independent regulation of matrix metalloproteinases and plasminogen activators in human fibrosarcoma cells

Journal of Cellular Physiology
Y T LimY A DeClerck

Abstract

Serine proteases and matrix metalloproteinases have been shown to often cooperate in multiple physiological and pathological processes associated with changes in the extracellular matrix (ECM). We have examined the interaction between the plasminogen activator (PA)-plasmin system and matrix metalloproteinases (MMPs) in HT1080 human fibrosarcoma cells treated with 12-O-tetradecanoyl-phorbol-13-acetate (TPA). While TPA treatment evoked a temporary increased expression of urokinase type PA (uPA), the production of both types of human plasminogen activator inhibitors (PAI) was induced and sustained over 12 h by TPA treatment shifting the protease-protease inhibitors balance in favor of the inhibitors. TPA treatment of HT1080 cells induced the expression of interstitial collagenase (MMP-1) and increased the expression of gelatinase B (MMP-9), tissue inhibitor of metalloproteinases-1 (TIMP-1), and MT-MMP, a membrane-bound activator of progelatinase A (proMMP-2), while MMP-2 and TIMP-2 expression were decreased. Increased MT-MMP expression by TPA treatment was associated with increased activation of proMMP-2. These data show that the regulation of PA-plasmin and metalloproteinase and their specific inhibitors is uncoordinated. In addi...Continue Reading

References

Nov 1, 1992·Cancer Metastasis Reviews·H C Kwaan
Jul 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L M Matrisian
Aug 1, 1992·American Journal of Respiratory Cell and Molecular Biology·G Murphy, A J Docherty
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H E Van Wart, H Birkedal-Hansen
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C S HEG I Goldberg
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G I GoldbergC S He
Mar 1, 1985·Collagen and Related Research·H G WelgusG P Stricklin
Jan 1, 1988·Enzyme·E K Kruithof
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y A DeClerck, S Imren
Oct 1, 1993·Current Opinion in Cell Biology·D E Kleiner, W G Stetler-Stevenson
Oct 1, 1993·Chest·S T Weiss
Feb 1, 1993·International Journal of Cancer. Journal International Du Cancer·M HoudeG Opdenakker

❮ Previous
Next ❯

Citations

Jul 23, 1999·Annals of the New York Academy of Sciences·L JonesJ P Neoptolemos
Apr 4, 2008·Investigative Ophthalmology & Visual Science·Budd TuckerMichael J Young
Jul 8, 2003·The Journal of Pathology·Sylvia HeenemanMat J Daemen
Apr 17, 2008·Biochemical and Biophysical Research Communications·Joong Sup ShimHo Jeong Kwon
Aug 3, 2007·Matrix Biology : Journal of the International Society for Matrix Biology·Hyun-Jeong Ra, William C Parks
Oct 19, 1999·Thyroid : Official Journal of the American Thyroid Association·J W SmitB M Goslings
Oct 11, 1996·The Journal of Biological Chemistry·K HammaniY A DeClerck
Feb 1, 1997·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·J H UhmV W Yong
Nov 14, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Boris I RatnikovAlex Y Strongin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.