Independent regulation of thromboxane and prostaglandin synthesis in liver macrophages

Biochemical Pharmacology
P DieterK Decker

Abstract

Incubation of liver macrophages with zymosan, phorbol ester and calcium ionophore A 23187 led to the formation of thromboxane, prostaglandin E2 and prostaglandin D2, whereas after external addition of arachidonic acid prostaglandin E2 and prostaglandin D2 only were found. This was confirmed by the use of labeled arachidonic acid given together with the stimuli. When the liver macrophages were prelabeled with [3H]arachidonic acid, and zymosan and [14C]arachidonic acid were added simultaneously, [3H]-label only was found in thromboxane whereas both [3H]- and [14C]-labeled PGE2 and PGD2 were detected in the cell medium. These data suggest that in cultured rat liver macrophages externally added arachidonic acid is accessible to the cyclooxygenase supplying prostaglandin H2 for prostaglandin E2 and D2 synthesis but not for thromboxane synthesis.

References

Oct 15, 1988·European Journal of Biochemistry·P DieterK Decker
Sep 1, 1987·Prostaglandins·U F SchadeE T Rietschel
May 15, 1986·European Journal of Biochemistry·M HeckerS Terao
Apr 2, 1984·European Journal of Biochemistry·M BirmelinK Decker

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Citations

Sep 2, 2000·Journal of Biochemical and Molecular Toxicology·A M DerbocioE L Ishii-Iwamoto
Sep 14, 1994·Journal of Immunological Methods·P Dieter
Sep 11, 1990·European Journal of Biochemistry·K Decker
Mar 26, 2005·Prostaglandins & Other Lipid Mediators·Sabine PestelHenrike L Schieferdecker
Feb 15, 2000·International Reviews of Immunology·J T Attwood, D H Munn
Apr 16, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sabine PestelHenrike L Schieferdecker

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