Independent roles of eIF5A and polyamines in cell proliferation

The Biochemical Journal
Kazuhiro NishimuraK Igarashi

Abstract

To examine the roles of active hypusinated eIF5A (eukaryotic translation initiation factor 5A) and polyamines in cell proliferation, mouse mammary carcinoma FM3A cells were treated with an inhibitor of deoxyhypusine synthase, GC7 (N1-guanyl-1, 7-diaminoheptane), or with an inhibitor of ornithine decarboxylase, DFMO (a-difluoromethylornithine), or with DFMO plus an inhibitor of spermine synthase, APCHA [N1-(3-aminopropyl)-cyclohexylamine]. Treatment with GC7 decreased the level of active eIF5A on day 1 without affecting cellular polyamine content, and inhibition of cell growth occurred from day 2. This delay reflects the fact that eIF5A was present in excess and was very stable in these cells. Treatment with DFMO or with DFMO plus APCHA inhibited cell growth on day 1. DFMO considerably decreased the levels of putrescine and spermidine, and the formation of active eIF5A began to decrease when the level of spermidine fell below 8 nmol/mg of protein after 12 h of incubation with DFMO. The combination of DFMO and APCHA markedly decreased the levels of putrescine and spermine and significantly decreased the level of spermidine, but did not affect the level of active eIF5A until day 3 when spermidine level decreased to 7 nmol/mg of pr...Continue Reading

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Citations

Mar 24, 2006·Amino Acids·K IgarashiK Kashiwagi
Aug 19, 2011·Amino Acids·Kazuhiro NishimuraMyung Hee Park
Dec 6, 2011·Amino Acids·M CaragliaA Abbruzzese
Sep 10, 2005·Apoptosis : an International Journal on Programmed Cell Death·M SchillerH M Lorenz
Mar 15, 2012·Investigational New Drugs·Patrick ZieglerTim H Brümmendorf
Apr 29, 2011·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Bhavna Chawla, Rentala Madhubala
Mar 19, 2013·The International Journal of Biochemistry & Cell Biology·Tomoko YamashitaKazuei Igarashi
May 3, 2008·Proceedings of the National Academy of Sciences of the United States of America·Manas K ChattopadhyayHerbert Tabor
May 27, 2010·The Journal of Clinical Investigation·Bernhard MaierRaghavendra G Mirmira
Dec 22, 2006·BMC Genomics·Diane M SpurlockLauren M McIntyre
Dec 6, 2008·BMC Physiology·Paul Vigne, Christian Frelin
Sep 12, 2013·SpringerPlus·Yurika NishikiBernhard Maier
Oct 31, 2009·Cell Structure and Function·Masahiro OhtaniTakami Oka
Nov 20, 2013·International Journal of Biological Sciences·Feng-wei WangDan Xie
Sep 12, 2013·Amino Acids·Tuomo A KeinänenAlex R Khomutov
Jan 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Swati MandalMyung Hee Park
Mar 25, 2014·Planta·Antonio F TiburcioRubén Alcázar
Feb 27, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sarah M FrancisJohn E Thompson
Jan 23, 2009·Journal of Cellular Physiology·Alexander GosslauKuang Yu Chen
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Apr 25, 2015·IUBMB Life·Kazuei Igarashi, Keiko Kashiwagi
Jun 23, 2009·Journal of Cellular and Molecular Medicine·Susanna VuohelainenLeena Alhonen
Sep 7, 2013·Biomolecules & Therapeutics·Myung Hee Park, Kazuei Igarashi
May 16, 2013·The Biochemical Journal·Mervi T HyvönenTuomo A Keinänen
Jun 5, 2013·Journal of Zhejiang University. Science. B·Guo-dong XuGuo-feng Shao
May 12, 2009·The International Journal of Biochemistry & Cell Biology·Kazuhiro NishimuraKazuei Igarashi
Aug 1, 2009·The International Journal of Biochemistry & Cell Biology·Kazuei Igarashi, Keiko Kashiwagi
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Simon IrmerDietrich Ober
Aug 2, 2011·Molecular and Biochemical Parasitology·Bertha Isabel Carvajal-GamezMaría Elizbeth Alvarez-Sánchez

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