PMID: 7540157Feb 1, 1995Paper

Independent suppression of nitric oxide and TNF alpha in the lung of conscious rats by ethanol

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
J XieS Greenberg

Abstract

Tumor necrosis factor-alpha (TNF alpha) and nitric oxide (NO) mediate in part the microbicidal response of murine and rodent alveolar macrophages (AM) and recruited neutrophils (PMN) to airborne infections. Ethanol (ETOH) suppresses intrapulmonary TNF alpha and NO release and impairs pulmonary host defense mechanisms. We tested the concept that ETOH down-regulates NO by inhibiting production of TNF alpha. Male rats were given intratracheal (i.t.) saline (PBS), a polyclonal anti-TNF alpha antibody (TNFab) or nonimmune IgG (22 mg/kg, i.m.) 2 h before giving i.t. Escherichia coli endotoxin (LPS) to normal rats or rats pretreated with ETOM (5.5 g/kg, i.p.) 30 min before experimentation. AM and PMN were obtained from the bronchoalveolar lavage fluid (BAL) fluid of rats killed 2 and 4 h after administration of LPS. mRNA for inducible NO synthase (iNOS) and TNF alpha were measured in AM and PMN with competitor equalized RT-PCR techniques. The BAL fluid, AM, and PMN were assayed for TNF alpha and NO2-, and NO3- (RNI) with the L929 bioassay and chemiluminescence, respectively. TNFab abolished LPS-induced increases in TNF alpha but did not suppress the NO content of the BAL fluid or gene expression for iNOS by AM or PMN. ETOH suppressed ...Continue Reading

Citations

May 15, 2003·Addiction Biology·Hassan Vally, Philip J Thompson
Oct 26, 2005·Respiratory Research·Kejing SongJay K Kolls
Dec 31, 1997·The American Journal of Physiology·S S GreenbergJ Ouyang
Jan 5, 2000·American Journal of Respiratory and Critical Care Medicine·D A StoltzG J Bagby
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P SartiM Brunori
Oct 1, 1996·Alcoholism, Clinical and Experimental Research·S S GreenbergT D Giles
Mar 27, 2002·Nature Reviews. Immunology·Steve Nelson, Jay K Kolls
Feb 1, 1996·Alcoholism, Clinical and Experimental Research·N B D'SouzaI V Deaciuc
Nov 9, 2010·Human & Experimental Toxicology·Lucija SarcMetoda Lipnik-Stangelj
Apr 11, 2014·American Journal of Rhinology & Allergy·Ju Wan KangJeong Hong Kim
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xue-Jun ZhaoJay K Kolls
Apr 16, 1999·British Journal of Pharmacology·P J SyapinJ D Militante
Dec 31, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Cuihua ZhangWilliam M Chilian
Sep 10, 1998·Nitric Oxide : Biology and Chemistry·S S GreenbergT D Giles
Sep 26, 1997·Alcohol·I V Deaciuc

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