Independently silencing two photosynthetic proteins in Nicotiana attenuata has different effects on herbivore resistance.

Plant Physiology
Sirsha Mitra, Ian T Baldwin

Abstract

Insect attack frequently down-regulates photosynthetic proteins. To understand how this influences the plant-insect interaction, we transformed Nicotiana attenuata to independently silence ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase) activase (RCA) and RuBPCase and selected lines whose photosynthetic capacity was similarly reduced. Decreases in plant growth mirrored the decreases in photosynthesis, but the effects on herbivore performance differed. Both generalist (Spodoptera littoralis) and specialist (Manduca sexta) larvae grew larger on RCA-silenced plants, which was consistent with decreased levels of trypsin protease inhibitors and diterpene glycosides and increased levels of RuBPCase, the larvae's main dietary protein. RCA-silenced plants were impaired in their attack-elicited jasmonate (JA)-isoleucine (Ile)/leucine levels, but RuBPCase-silenced plants were not, a deficiency that could not be restored by supplementation with Ile or attributed to lower transcript levels of JAR4/6, the key enzyme for JA-Ile conjugation. From these results, we infer that JA-Ile/leucine signaling and the herbivore resistance traits elicited by JA-Ile are influenced by adenylate charge, or more generally, carbon availability in R...Continue Reading

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Citations

Sep 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Venkatesan RadhikaWilhelm Boland
Dec 22, 2010·Plant Physiology·Xiaoyi ShanDaoxin Xie
Aug 27, 2015·The Plant Journal : for Cell and Molecular Biology·Zhihao LingShuqing Xu
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