Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease

Rice
Qian SunYuan Hu Xuan

Abstract

Loose Plant Architecture 1 (LPA1), an indeterminate domain (IDD) protein, exhibits almost no expression in the leaves, but the overexpression of LPA1 significantly increases the resistance of rice to sheath blight disease (ShB) via the activation of PIN-FORMED 1a (PIN1a). In this study, we determined that Rhizoctonia solani infection significantly induced LPA1 expression in the leaves, and lpa1 was more susceptible to R. solani compared with the wild-type and revertant plants. In addition, infection with R. solani altered the expression of IDD3, IDD5, IDD10, and IDD13, and yeast two-hybrid, split-GFP, and coimmunoprecipitation assays showed that LPA1 interacts with IDD3 and IDD13. IDD13 RNAi plants were more susceptible, while IDD13 overexpressors were less susceptible to ShB compared with the wild-type. In parallel, idd3 exhibited no significant differences, while IDD3 overexpressors were more susceptible compared to the wild-type response to ShB. Additional chromatin-immunoprecipitation and electrophoretic mobility shift assay experiments indicated that IDD13 and IDD3 bound to the PIN1a promoter, and the transient assay indicated that IDD13 and IDD3 positively and negatively regulate PIN1a expression, respectively. Moreover, ...Continue Reading

References

Mar 17, 2004·Nucleic Acids Research·Akiko KozakiJoseph Colasanti
Dec 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Zhongying ChenBarbara N Kunkel
Sep 9, 2008·The Plant Journal : for Cell and Molecular Biology·Soon Ju ParkChang-deok Han
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Mar 20, 2010·Cold Spring Harbor Perspectives in Biology·Eva ZazímalováPetr Hosek
Jan 27, 2011·The Plant Journal : for Cell and Molecular Biology·Pil Joon SeoChung-Mo Park
Jun 15, 2011·Annual Review of Phytopathology·Alexandre Robert-SeilaniantzJonathan D G Jones
Jan 3, 2013·The New Phytologist·Yuan Hu XuanChang-Deok Han
Jan 22, 2015·The Plant Cell·Maciek Adamowski, Jiří Friml
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Methods Mentioned

BETA
co-immunoprecipitation
co-IP
two-hybrid
immunoprecipitation
ChIP
transgenic
ChIP-PCR
electrophoretic mobility shift assay
electrophoretic
PCR

Software Mentioned

Image J Fiji
GraphPad
Prism

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