Abstract
Hemidesmus indicus (L.) R. Br. ex Schult is commonly known as anantmul or Indian sarsaparilla. The roots of this plant, which display a wide range of medicinal, biological, and phytopharmaceutical properties, are used in the pharmaceutical and food industries. Conventionally, the plant is propagated by seed germination or vegetatively, but the efficacy of traditional methods has some limitations: plants derived from seed germination are prone to seed-borne diseases, or plantlet production using vegetative propagation is limited. In contrast, plant tissue culture allows for large-scale propagation and secondary metabolite production in vitro without sacrificing plants from their natural habitats. Many efforts have been made over 40 years of research to establish efficient micropropagation protocols to speed up cultivation of this plant, including callus-mediated in vitro propagation, somatic embryogenesis, and shoot multiplication using cotyledenory nodes, stem segments, shoot tips, and nodal explants. Among these explants, nodal explants are the most commonly used for H. indicus micropropagation. The application of adenine sulfate, citric acid, ascorbic acid, and arginine may be useful in preventing explant browning, premature ...Continue Reading
References
Apr 1, 1968·Experimental Cell Research·O L GamborgK Ojima
Jul 1, 1934·Plant Physiology·P R White
Jan 3, 1969·Science·J P Nitsch, C Nitsch
Nov 21, 2007·Journal of Plant Physiology·Dipjyoti ChakrabortyAdinpunya Mitra
May 23, 2012·Plant Physiology and Biochemistry : PPB·Anish KunduAdinpunya Mitra
Oct 6, 2012·Biotechnology Advances·Shiwali SharmaJaime A Teixeira da Silva
Dec 6, 2012·Molecular Biology Reports·Mehwish YaseenIshfaq Ahmad Hafiz
Feb 1, 1996·Plant Cell Reports·J Patnaik, B K Debata
May 5, 2016·Development·Momoko IkeuchiKeiko Sugimoto
Jan 1, 2016·3 Biotech·Hare KrishnaRadha Kishan Sadh
May 8, 2018·Planta·Claudia A Espinosa-LealSilverio García-Lara
Jun 1, 2018·Oncotarget·Eleonora TurriniCarmela Fimognari
May 12, 2019·Scientific Reports·Eleonora TurriniCarmela Fimognari
Jun 16, 2019·Journal of Ethnopharmacology·Snehal SuryavanshiRuchika Kaul-Ghanekar
Aug 1, 2019·Applied Microbiology and Biotechnology·Eashan MukherjeeSomnath Bhattacharyya
Jan 29, 2020·Applied Microbiology and Biotechnology·Monisha MitraNirmal Mandal
Feb 3, 2020·Journal of Ethnopharmacology·Samapika NandyAbhijit Dey