Indication for thresholds of chromosome non-disjunction versus chromosome lagging induced by spindle inhibitors in vitro in human lymphocytes

Mutagenesis
A ElhajoujiM Kirsch-Volders

Abstract

Risk assessment from exposure to spindle inhibitors should take into account the possibility of threshold concentration-response curves for aneuploidy induction. We analysed concentration-dependent induction of chromosome non-disjunction by well known spindle poisons (colchicine, carbendazim, mebendazole and nocodazole) and a reference clastogen, methyl methanesulphonate (MMS) in vitro in human lymphocytes; and integrated these findings with earlier results of chromosome loss in micronuclei. Chromosome non-disjunction was estimated on cytokinesis-blocked lymphocytes after simultaneous fluorescent in situ hybridization labelling with two chromosome-specific centromeric probes (chromosomes 1 and 17). The frequencies of spontaneous non-disjunction showed important inter-individual variations and were surprisingly high (7.04-15.39%). Lower concentrations of aneugens did not induce a statistically significant increase of non-disjunction frequencies over the respective control levels, whereas higher concentrations clearly induced a concentration-dependent increase in the non-disjunction frequencies with the four aneugens tested. On the contrary, even at high concentrations, MMS induced a slight increase in the frequency of non-disjun...Continue Reading

Citations

Mar 10, 2001·Genes, Chromosomes & Cancer·A G de Nooij-Van DalenM Giphart-Gassler
Oct 11, 2003·Mutation Research·Micheline Kirsch-VoldersAkihiro Wakata
Dec 13, 2000·Mutation Research·M Fenech
Jul 11, 2002·Mutation Research·Micheline Kirsch-VoldersIlse Decordier
Apr 5, 2003·Toxicology Letters·Iona S Pratt, Thomasina Barron
Apr 5, 2003·Toxicology Letters·Micheline Kirsch-VoldersIlse Decordier
Sep 26, 2002·Mutation Research·Sara FriasDora Gilda Mayén
Jan 14, 2000·Mutation Research·M Kirsch-VoldersA Elhajouji
Jan 15, 2000·Mutation Research·R Santos-MelloA Almeida
Jun 5, 2007·Nature Protocols·Michael Fenech
Feb 23, 2007·Molecular Biology of the Cell·Marie A JanickeJames R LaFountain
Jan 5, 2008·Carcinogenesis·Julie MullerMicheline Kirsch-Volders
May 31, 2011·Environmental Health Perspectives·Kim Vande LoockMicheline Kirsch-Volders
May 16, 2013·PloS One·Lya G HernándezGeorge E Johnson
May 7, 2010·Analytical and Bioanalytical Chemistry·Valérie Fessard, Ludovic Le Hégarat
Dec 18, 2010·Mutagenesis·Azeddine ElhajoujiMicheline Kirsch-Volders
Jan 15, 2016·Environmental and Molecular Mutagenesis·Steven M BryceStephen D Dertinger
Jan 29, 2003·Environmental and Molecular Mutagenesis·Sophie MeintièresDaniel Marzin
Feb 24, 2001·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·M A AkbarshaA Faridha
Jul 27, 2010·Mutation Research·James M Parry, Micheline Kirsch-Volders
Nov 7, 2002·Reproductive Biomedicine Online·Ursula Eichenlaub-RitterHans-Rudolf Tinneberg
May 19, 2010·Mutation Research·Ilse DecordierMicheline Kirsch-Volders
Nov 26, 2009·Toxicology·Gareth J S JenkinsShareen H Doak
May 27, 2009·Mutation Research·George E JohnsonGareth J Jenkins
May 16, 2009·Mutation Research·M Kirsch-VoldersD Kirkland
Apr 28, 2009·Mutation Research·Prashantha Naik, K K Vijayalaxmi

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