Indinavir inhibits the expression of cytoplasmic aromatase and nuclear SREBP in the hippocampus of reperfusion injury-induced ischemic rats

The Journal of Steroid Biochemistry and Molecular Biology
Mehtap CincioğluPelin Kelicen-Ugur

Abstract

Ischemic brain injury due to insults, such as stroke, is a leading cause of severe neurological and neurobehavioral deficits and death. Neurodegeneration can be prevented by local aromatase expression, and estrogen synthesis can be neuroprotective in ischemia/reperfusion. Therefore, aromatase, the enzyme that transforms androgens to estrogens, may be a potential target for the study of reperfusion injury after brain ischemia. We investigated the expression of aromatase and sterol regulatory element binding protein (SREBP) using Western blotting in the rat hippocampus after transient global ischemia plus hypotension. After 10 min of ischemia, aromatase and SREBP expression was observed in cytosolic extracts after 1, 4 and 10 weeks of reperfusion. Previous immunoblot analysis demonstrated that the highest aromatase expression appeared in damaged hippocampi after 1 week. In this study, SREBP expression was increased at 1 week in the nuclear extracts of damaged hippocampi. The aromatase inhibitor megestrol acetate (20 mg/kg/day; 21 days) and the SREBP inhibitor indinavir (15 mg/kg/day; 30 days) suppressed aromatase levels in ischemic hippocampi. Our findings indicate that ischemia, as well as chronic neurodegenerative processes, le...Continue Reading

References

Oct 1, 1990·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·G M PadillaV Petrow
Jan 1, 1967·Acta Obstetricia Et Gynecologica Scandinavica·P E Lebech, E L Nordentoft
Jan 13, 1994·The New England Journal of Medicine·G S TintG Salen
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M SimonsK Simons
Jan 30, 1999·Psychoneuroendocrinology·E E Baulieu
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M S Brown, J L Goldstein
Nov 24, 1999·The Journal of Clinical Endocrinology and Metabolism·B ZhangD E Moller
Dec 10, 1999·The Journal of Biological Chemistry·G SchmidtkeM Groettrup
May 12, 2000·The Journal of Biological Chemistry·H MurataM Mueckler
Oct 21, 2000·Progress in Neurobiology·L M Garcia-SeguraL L DonCarlos
May 15, 2001·Journal of Neurobiology·I AzcoitiaL M Garcia-Segura
Feb 19, 2002·The Journal of Steroid Biochemistry and Molecular Biology·M E JonesE R Simpson
Jul 20, 2002·Trends in Endocrinology and Metabolism : TEM·Phyllis M Wise
Aug 6, 2002·Molecular and Cellular Endocrinology·Y MurataE R Simpson
Dec 4, 2002·Nature Reviews. Neuroscience·Anne E WestMichael E Greenberg
Mar 27, 2003·Nature Neuroscience·Luigi PuglielliDora M Kovacs
Jul 29, 2003·Aging Cell·Iñigo AzcoitiaLuis M Garcia-Segura
Sep 3, 2003·Biochemical and Biophysical Research Communications·Changman ZhouJohn H Zhang
Mar 1, 1964·The Medical Clinics of North America·J W GOLDZIEHER
Mar 6, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Isabelle SponneThierry Pillot
Mar 8, 2005·Journal of Neurochemistry·Abedelnasser AbulrobDanica Stanimirovic
Apr 23, 2005·Glia·Rona G Giffard, Raymond A Swanson
May 18, 2005·The Journal of Steroid Biochemistry and Molecular Biology·H V O CarswellI M Macrae
Jun 28, 2005·Lancet·Valery L Feigin
Feb 17, 2006·Trends in Endocrinology and Metabolism : TEM·Margaret E E JonesEvan R Simpson
May 25, 2006·Current Topics in Medicinal Chemistry·Michal Hetman, Giorgi Kharebava
Jun 13, 2006·Cellular and Molecular Neurobiology·Pelin Kelicen, Agneta Nordberg
Jul 1, 2006·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Bo JiangTammy R Dugas
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Rao Muralikrishna AdibhatlaJames Franklin Hatcher
Nov 15, 2007·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Sofia Papadia, Giles E Hardingham
May 13, 2008·Lancet·Geoffrey A DonnanStephen M Davis

❮ Previous
Next ❯

Citations

Jun 27, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Asadollah MohammadiMarzieh Tahavor
Nov 19, 2019·Translational Neuroscience·Pelin Kelicen-UgurHande Karahan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acute Stroke

A stroke occurs when blood supply to the brain is interrupted depriving the brain of oxygen and nutrients. This feed focuses cerebrovascular accidents including ischemic and paralytic stroke.

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Carotid Artery Diseases

Carotid artery disease is a group of pathological conditions of the carotid artery. Discover the latest research on carotid artery disease here.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Atrial Filbrillation

Atrial fibrillation refers to the abnormal heart rhythm characterized by rapid and irregular beating of the atria. Here is the latest research.