Indirect 3D bioprinting and characterization of alginate scaffolds for potential nerve tissue engineering applications

Journal of the Mechanical Behavior of Biomedical Materials
Saman NaghiehXiongbiao Chen

Abstract

Low-concentration hydrogels have favorable properties for many cell functions in tissue engineering but are considerably limited from a scaffold fabrication point of view due to poor three-dimensional (3D) printability. Here, we developed an indirect-bioprinting process for alginate scaffolds and characterized the potential of these scaffolds for nerve tissue engineering applications. The indirect-bioprinting process involves (1) printing a sacrificial framework from gelatin, (2) impregnating the framework with low-concentration alginate, and (3) removing the gelatin framework by an incubation process, thus forming low-concentration alginate scaffolds. The scaffolds were characterized by compression testing, swelling, degradation, and morphological and biological assessment of incorporated or seeded Schwann cells. For comparison, varying concentrations of alginate scaffolds (from 0.5% to 3%) were fabricated and sterilized using either ultraviolet light or ethanol. Results indicated that scaffolds can be fabricated using the indirect-bioprinting process, wherein the scaffold properties are affected by the concentration of alginate and sterilization technique used. These factors provide effective means of regulating the propertie...Continue Reading

Citations

Aug 11, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Carmen C Piras, David K Smith
May 22, 2020·Journal of Polymers and the Environment·Alina GhilanVlad Mihai Chiriac
Oct 5, 2019·Materials·Natasha Antill-O'BrienCathal D O'Connell
Nov 18, 2020·Advanced Healthcare Materials·Melissa CadenaSteven A Sloan
Feb 11, 2021·Biomaterials Science·Harshavardhan BudharajuSwaminathan Sethuraman
Feb 18, 2021·Tissue Engineering. Part B, Reviews·Omar A SelimDeepak M Kalaskar
Feb 23, 2021·International Journal of Biological Macromolecules·Chuhuan HuYapeng Fang
Aug 12, 2020·Current Drug Delivery·Dhalendra KothaleDharmendra Jain
Mar 30, 2021·Journal of Tissue Engineering and Regenerative Medicine·Leila RoshangarAhad Ferdowsi Khosroshahi
Feb 19, 2021·Biofabrication·Zhouquan FuXiongbiao Chen
May 1, 2021·Pharmaceuticals·Paula Gabriela ManitaManoli Igartua
May 24, 2021·Advances in Colloid and Interface Science·Shadpour MallakpourChaudhery Mustansar Hussain
Jul 3, 2021·Burns and Trauma·Yulan HuangMaling Gou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.