Indirect calculation of monoclonal antibodies in nanoparticles using the radiolabeling process with technetium 99 metastable as primary factor: Alternative methodology for the entrapment efficiency

Journal of Pharmaceutical and Biomedical Analysis
Edward Helal-NetoRalph Santos-Oliveira

Abstract

The use of monoclonal antibodies (Mab) in the current medicine is increasing. Antibody-drug conjugates (ADCs) represents an increasingly and important modality for treating several types of cancer. In this area, the use of Mab associated with nanoparticles is a valuable strategy. However, the methodology used to calculate the Mab entrapment, efficiency and content is extremely expensive. In this study we developed and tested a novel very simple one-step methodology to calculate monoclonal antibody entrapment in mesoporous silica (with magnetic core) nanoparticles using the radiolabeling process as primary methodology. The magnetic core mesoporous silica were successfully developed and characterised. The PXRD analysis at high angles confirmed the presence of magnetic cores in the structures and transmission electron microscopy allowed to determine structures size (58.9 ± 8.1 nm). From the isotherm curve, a specific surface area of 872 m2/g was estimated along with a pore volume of 0.85 cm3/g and an average pore diameter of 3.15 nm. The radiolabeling process to proceed the indirect determination were well-done. Trastuzumab were successfully labeled (>97%) with Tc-99m generating a clear suspension. Besides, almost all the Tc-99m u...Continue Reading

Citations

Nov 20, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Filipe Leal PortilhoRalph Santos-Oliveira
Aug 20, 2019·Current Pharmaceutical Design·Julio Cezar de Almeida JuniorRalph Santos-Oliveira
Aug 31, 2020·Dose-response : a Publication of International Hormesis Society·Guichen LiDongming Wang
Jan 26, 2021·Chemical Society Reviews·Juan PellicoRafael T M de Rosales
Jul 30, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hongmei ZengSixiang Wang

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