Indirect coating of RGD peptides using a poly-L-lysine spacer enhances jaw periosteal cell adhesion, proliferation, and differentiation into osteogenic tissue

Journal of Biomedical Materials Research. Part a
Nina ArdjomandiDorothea Alexander

Abstract

The aim of our study was to generate a biofunctionalized, three-dimensional (3D) biomaterial to enhance jaw periosteal cell (JPC) adhesion and differentiation into osteogenic tissue. Therefore, open-cell polylactic acid (OPLA) scaffolds were coated covalently with different RGD peptides (a conserved recognition sequence of the most ECM proteins--arginine-glycine-asparagine) and different coating variants. The linear and cyclic RGD peptides were either applied directly or indirectly via a poly-L-lysine (PLL) spacer. JPCs were analyzed on coated constructs in 2D and 3D cultures and showed enhanced rates for indirectly coated scaffolds using the PLL spacer. By gene expression, we detected significantly increased levels of osteogenic marker genes, such as alkaline phosphatase, RUNX2, and AMELY in JPCs seeded onto PLL/linear RGD constructs compared to the otherwise-coated constructs. An analysis of the JPC mineralization capacity revealed the highest amounts of calcium-phosphate precipitates in cells growing within the PLL/linear scaffolds. Additionally, the JPC adhesion behavior on OPLA scaffolds seems to be mediated by ITGB3, ITGB1, and ITGAV, as shown by blocking assays. We concluded that coating of OPLA constructs with linear RG...Continue Reading

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Citations

May 18, 2013·Journal of Biomaterials Science. Polymer Edition·Jie ZhuXiaolin Zhu
Dec 12, 2013·Journal of Materials Science. Materials in Medicine·J NiederlaenderHans Peter Wendel
Jul 4, 2015·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·N ArdjomandiS Sauerbier
Jan 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yang LiuCleo Choong
Mar 9, 2021·Wiley Interdisciplinary Reviews. RNA·Mingyuan ZouJian Xu
Aug 17, 2017·ACS Applied Materials & Interfaces·Adina BadeaRalph G Nuzzo

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