PMID: 15348536Sep 7, 2004Paper

Indirect cytotoxicity evaluation of pseudowollastonite

Journal of Materials Science. Materials in Medicine
D DufraneM Anseau

Abstract

This study aimed to evaluate the cytotoxicity of substances leached by pseudowollastonite (CaSiO(3)). It has been previously shown that calcium (Ca(2+)) and silicate (SiO(3)(-)) ions are released from pseudowollastonite into biological solutions. Both of these ions are known to influence the biological metabolism of osteoblastic cells essential in the mineralization process and bone-bonding mechanism. The indirect toxicity evaluation was performed by extraction method, according to International Standard Organization (ISO). Pseudowollastonite pellets obtained by solid-state reaction were incubated, in culture medium, during 24, 48, 72 or 168 h at different concentrations (5, 10, 15, 50, 100, 200 mg/ml). The cytotoxicity of each extract in presence of human osteoblastic cell line (SaOS-2) was quantitatively assessed by measuring the viability (succinate dehydrogenase activity, MTT), the membrane integrity (the uptake of the neutral red by viable cells, NR) as well as the cell necrosis by measuring the lactate dehydrogenase (LDH) released in the culture medium. No significant alteration of membrane integrity or cell suffering was detectable. However, increased cell metabolism was observed for cells exposed to pseudowollastonite e...Continue Reading

Citations

May 16, 2007·Journal of Biomedical Materials Research. Part a·R G CarrodeguasS De Aza
Jun 1, 2018·The Journal of International Medical Research·Marcelo Sanmartin de AlmeidaJosé Mauro Granjeiro
Apr 2, 2004·Journal of Biomedical Materials Research. Part a·Carlos SarmentoIan J McKay
Mar 2, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Piedad N De AzaMichel Anseau
Jul 30, 2011·Journal of Tissue Engineering and Regenerative Medicine·Luis M Rodriguez-LorenzoJulio San Román
Feb 22, 2011·Journal of Materials Science. Materials in Medicine·Miguel A de la Casa-LilloPiedad N De Aza
May 7, 2008·Biomedical Materials·Jun OuGuangfu Yin
Dec 23, 2004·Journal of Biomaterials Applications·Li ZhaoWanyin Zhai
Mar 11, 2017·Journal of Biomedical Materials Research. Part a·Xianfeng ZhouNita Sahai
Aug 25, 2018·Journal of Applied Biomaterials & Functional Materials·Kazem MarzbanSara Bagherifard
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Natalia Pajares-ChamorroXanthippi Chatzistavrou
Oct 26, 2005·Inorganic Chemistry·Michel R AnseauThomas W Swaddle

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