PMID: 2496168Apr 21, 1989Paper

Indirect double sandwich ELISA for the specific and quantitative measurement of mouse IgM, IgA and IgG subclasses

Journal of Immunological Methods
A S Klein-SchneegansF Loor

Abstract

We have developed sensitive enzyme-linked immunosorbent assays (ELISA) which measure mouse serum heavy chain immunoglobulin isotypes in nanograms per milliliter. In each case the specific isotypic Ig is sandwiched between an isotype-specific antibody used for coating and another isotype-specific antibody coupled to biotin for detection (with alkaline phosphatase coupled to avidin). These methods are simple to perform, specific for each isotype, reproducible with an average coefficient of variation of 5% for IgG1, 3% for IgG2a, 7% for IgG2b, 10% for IgG3, 3% for IgA and 7% for IgM, and at least 100 times more sensitive than radial immunodiffusion. The assays have been used to determine the absolute concentrations of mouse serum heavy chain Ig isotypes.

References

Nov 1, 1985·The Journal of Infection·J M Echevarría
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans
Feb 25, 1983·Journal of Immunological Methods·P Lems-Van KanA S Peña
Feb 1, 1984·Immunology Today·M Wilchek, E A Bayer

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Citations

Mar 21, 1991·Journal of Immunological Methods·S Froidevaux, F Loor
Jan 27, 2007·The American Journal of Pathology·Toshikazu NishieMasahide Asano
Sep 2, 1999·Nature Medicine·F ZhengA B Mukherjee
Jul 26, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·F NogakiS Sasayama

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