PMID: 6995567Aug 1, 1980

Indirect enzyme-linked immunosorbent assay for the quantitation of the type-specific antigen of Haemophilus influenzae b: a preliminary report

The Journal of Pediatrics
J PeppleR H Yolken


Infections with Haemophilus influenzae type b are major causes of morbidity and mortality in young children. We developed an enzyme-linked immunosorbent assay for the diagnosis of Hib infections. The ELISA was more sensitive than both counterimmunoelectrophoresis and latex agglutination for the detection of Hib antigen in serum, urine, and cerebrospinal fluid. In addition, no false positive ELISA reactions were noted. The increased sensitivity of ELISA was especially valuable in the detection of Hib antigen following antibiotic therapy, in which case cultures are often sterile and CIE and LA can be negative. ELISA is thus a valuable tool for the early diagnosis of Hib infections.


Nov 1, 1985·Diagnostic Microbiology and Infectious Disease·M A Gerber
Mar 1, 1986·Annals of Emergency Medicine·J M Dougherty, J Jones
Mar 1, 1986·Diagnostic Microbiology and Infectious Disease·D M GranoffK L Cates
Jul 28, 1983·The American Journal of Medicine·S L Kaplan
Feb 1, 1989·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·O G Brakstad, J A Maeland
Jan 1, 1984·Annals of the New York Academy of Sciences·R H Yolken
Jan 1, 1983·Annals of the New York Academy of Sciences·R H YolkenM J Mears
Jan 1, 1990·Scandinavian Journal of Infectious Diseases. Supplementum·M A Salih

Related Concepts

Antigens, Bacterial
Countercurrent Electrophoresis Measurement
Enzyme-Linked Immunosorbent Assay
Haemophilus Infections
Haemophilus influenzae
Peroxidase-Antiperoxidase Complex Technique
Latex Fixation Tests

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