PMID: 9631927Jun 19, 1998Paper

Indirect estimation of the unbound fraction of cyclosporine in plasma

Therapeutic Drug Monitoring
F AkhlaghiK F Brown

Abstract

The unbound fraction (fU) of cyclosporine in plasma is approximately 0.02. The measurement of cyclosporine fU requires a laborious equilibrium dialysis procedure, which is not practical in a clinical setting. A mathematical model was developed to estimate cyclosporine fU from concentrations of serum lipoproteins, the major binding proteins for cyclosporine. Values of fU were determined ex vivo in 126 plasma samples obtained from 58 recipients of heart and lung transplants, using equilibrium dialysis. Concentrations of serum lipids, measured using standard enzymatic techniques, were used as concentration markers for serum lipoproteins. Patients were randomly assigned to either of two equal-sized groups. One group (subgroup 1) was used to evaluate the parameters of the model, and the other group (subgroup 2) was used to examine its predictive performance. The parameters were estimated using least squares non-linear regression. A model incorporating concentrations of serum HDL- and LDL-cholesterol, serum albumin, and time after transplantation gave the best fit. For subgroup 2, mean prediction error (ME), a measure of bias, and root mean squared error (RMSE) and median absolute error (MAE), measures of precision, and their 95% con...Continue Reading

References

Apr 1, 1978·Journal of Pharmacokinetics and Biopharmaceutics·K YamaokaT Uno
Aug 1, 1986·The Journal of Pharmacy and Pharmacology·D SgoutasI Jerkunica
May 1, 1987·The Journal of Pharmacy and Pharmacology·S Henricsson
Aug 1, 1981·Journal of Pharmacokinetics and Biopharmaceutics·L B Sheiner, S L Beal
Sep 7, 1995·The New England Journal of Medicine·A KeoghA Kaan
Jun 1, 1993·Clinical Pharmacokinetics·P du SouichS Erill
Dec 1, 1995·Therapeutic Drug Monitoring·A Lindholm

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Citations

Jul 20, 2002·Clinical Pharmacokinetics·Fatemeh Akhlaghi, Andrew K Trull
Sep 24, 2004·Therapeutic Drug Monitoring·Hamim ZahirAndrew J McLachlan
Nov 27, 2002·Therapeutic Drug Monitoring·Leire de la FuenteElena Suárez
Mar 2, 1999·Therapeutic Drug Monitoring·F AkhlaghiK Brown

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