Indirect evidence for the presence of non-specific anion channels in rabbit mandibular salivary gland acinar cells.

The Journal of Physiology
P D BrownK R Lau

Abstract

1. Intracellular pH (pHi) was measured using the fluorescent pH-sensitive dye 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein in acini isolated from the rabbit mandibular salivary gland. 2. Stimulation of the acinar cells with acetylcholine (ACh) evoked an intracellular acidosis, the size of which was dependent on the HCO3-concentration in the bathing medium. A half-maximal acidosis was observed at approximately 10 mM-HCO3-. ACh also evoked an acidosis in HCO3(-)-free solutions containing acetate; a half-maximal acidosis was observed at about 10 mM-acetate. 3. Propionate, lactate and butyrate were also able to support the ACh-evoked acidosis to varying extents. In contrast, formate, pyruvate and salicylate did not support the ACh-induced acidosis to any great extent. 4. Acetazolamide greatly reduced the size of the acidosis in HCO3(-)-buffered medium, but had no effect in acetate-buffered medium, suggesting that the inhibitory effect of acetazolamide was due to a specific inhibition of carbonic anhydrase activity. 5. The Cl- channel blockers diphenylamine-2-carboxylic acid (DPC, 1 mM) and 5-nitro-2-(3-phenylpropylamino)-benzoic acid (0.5 mM) abolished the ACh-evoked acidosis in both HCO3(-) -and acetate-buffered media. 6. The d...Continue Reading

Citations

Dec 2, 2010·Cell and Tissue Research·Eleni Roussa
Jan 1, 1994·Microscopy Research and Technique·K T IzutsuD E Johnson
Apr 1, 1995·Pflügers Archiv : European journal of physiology·P PoronnikD I Cook
Jun 1, 1994·Journal of Cellular Physiology·J C SeagraveJ R Martinez
Jan 1, 1996·Pflügers Archiv : European journal of physiology·K R LauR M Case
Nov 1, 1990·The American Journal of Physiology·M A GrayB E Argent
Jan 1, 1992·The American Journal of Physiology·T J Shuttleworth, C M Wood

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