Indirect fluorescent antibody testing of cerebrospinal fluid for diagnosis of equine protozoal myeloencephalitis

American Journal of Veterinary Research
Paulo C DuarteIan A Gardner

Abstract

To assess the use of CSF testing with an indirect fluorescent antibody test (IFAT) for diagnosis of equine protozoal myeloencephalitis (EPM) caused by Sarcocystis neurona. Test results of 428 serum and 355 CSF samples from 182 naturally exposed, experimentally infected, or vaccinated horses. EPM was diagnosed on the basis of histologic examination of the CNS. Probability distributions were fitted to serum IFAT results in the EPM+ and EPM-horses, and correlation between serum and CSF results was modeled. Pairs of serum-CSF titers were generated by simulation, and titer-specific likelihood ratios and post-test probabilities of EPM at various pretest probability values were estimated. Post-test probabilities were compared for use of a serum-CSF test combination, a serum test only, and a CSF test only. Post-test probabilities of EPM increased as IFAT serum and CSF titers increased. Post-test probability differences for use of a serum-CSF combination and a serum test only were < or = 19% in 95% of simulations. The largest increases occurred when serum titers were from 40 to 160 and pre-test probabilities were from 5% to 60%. In all simulations, the difference between pre- and post-test probabilities was greater for a CSF test only, ...Continue Reading

References

Feb 1, 1994·European Journal of Epidemiology·B DujardinP Van der Stuyft
Jan 1, 1993·Journal of Clinical Epidemiology·D L SimelD B Matchar
Dec 19, 1998·Journal of Veterinary Internal Medicine·J E FoleyN C Pedersen
Feb 24, 2001·The Veterinary Clinics of North America. Equine Practice·R J MacKayS M Reed
Jan 29, 2002·Journal of the South African Veterinary Association·A L LeisewitzL van Blerk
Jul 20, 2002·Journal of the American Veterinary Medical Association·Anne Grimsley CookJennifer K Morrow
Sep 27, 2002·Journal of Veterinary Internal Medicine·Martin FurrUNKNOWN American College of Veterinary Internal Medicine
Feb 13, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Paulo C DuarteIan A Gardner
Jul 9, 2004·Veterinary Research·Javier Castillo-Olivares, James Wood

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Citations

Jul 19, 2008·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Natalie D MylniczenkoAnn C Melli
Feb 3, 2007·Journal of the American Veterinary Medical Association·Monica AlemanSarah M Puchalski
Aug 21, 2007·Veterinary Parasitology·Hany M Elsheikha, Linda S Mansfield
Apr 27, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Carrie J FinnoNicola Pusterla

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