PMID: 16536053Mar 16, 2006Paper

Indirect immunofluorescence study on the cytoskeleton of normal (FG) and neoplastic (SGS/3A) cadmium treated fibroblasts

Italian Journal of Anatomy and Embryology = Archivio Italiano Di Anatomia Ed Embriologia
Roberto MadedduAlessio Pirino

Abstract

Cadmium is a heavy metal dangerous for the environment and for the human health, with well shown carcinogenic potentiality. Many studies have related the professional exposure of the Cadmium with human pulmonary, prostatic and renal tumors, and it would be a role also in the tumors of the liver, of the hemopoietic system, of the bladder and of the stomach. The aim of the current study is to examine in normal and neoplastic fibroblasts culture cells the modifications induced by the Cadmium at cellular level, in particular on the cytoskeleton, responsible not only of the intracellular transport of vesicles and cell organules, but also of their positioning and of the cellular integrity. Two fibroblastic cellular strains, normal (FG) and neoplastic (SGS/3A), have been incubated in a 5 microM Cadmium acetate added medium for 1, 8, 24 hours and studied by indirect immunofluorescence methods, particularly for the following proteins: Actin, Tubulin and Vimentin. The observations show in normal and neoplastic fibroblasts comparables modifications and anomalies of cytoskeletal shape. In both the cases the cellular morphology suffers drastic modifications, gradually evolving through intermediary shapes: from triangular and spindle-shaped ...Continue Reading

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