Indirect immunofluorescent staining of fibronectin associated with the floor of the foregut during formation and rupture of the oral membrane in the chick embryo

The Anatomical Record
R E Waterman, G Balian

Abstract

The distribution of the glycoprotein, fibronectin, within the cranial region of stage 8--16 chick embryos was examined by indirect immunofluorescence using paraffin sections exposed to affinity-purified rabbit anti-human CIG and FITC-conjugated goat anti-rabbit immunoglobulins. Fluorescence was present within the matrix surrounding the cranial mesenchyme, along the basal surfaces of all epithelia, and surrounding the notochord at all stages. Fluorescence associated with the floor of the foregut was particularly intense. The fluorescent layers beneath the ectoderm and endoderm of the oral (oropharyngeal) membrane at stage 8 merged into a single, continuous, intensely fluorescent line as the extracellular space within the oral membrane narrowed during stages 9--12. This line of uniform fluorescence parallels the previously described histological reorganization of the extracellular compartment of the oral membrane, but the ultrastructural localization of this fluorescent material remains unknown. Fluorescence was also intense beneath the foregut endoderm in the presumptive cardiac region caudal to the oral membrane and was continuous with strands of fluorescent material extending into the matrix of the dorsal mesocardium and cardi...Continue Reading

References

Dec 31, 1979·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J Wakely, M A England
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E J MacarakN A Kefalides
Jan 1, 1976·Developmental Biology·R O HynesC J Epstein
May 1, 1977·Journal of the National Cancer Institute·L A LiottaD Rynbrandt
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B R Zetter, G R Martin
Jun 20, 1978·Annals of the New York Academy of Sciences·M MatsudaK Wakabayashi
Mar 1, 1978·The Journal of Cell Biology·K HedmanJ Wartiovaara
Jun 1, 1978·The Journal of Experimental Medicine·E A Jaffe, D F Mosher
Apr 1, 1979·The Journal of Cell Biology·K HedmanM Höök
Jun 20, 1978·Annals of the New York Academy of Sciences·B R ZetterD Gospodarowicz
Apr 1, 1979·Developmental Biology·M KurkinenL Saxén
Feb 1, 1979·The Journal of Cell Biology·K M Yamada, D W Kennedy
Aug 1, 1979·The American Journal of Anatomy·G C Schoenwolf
Jan 1, 1977·Journal of Supramolecular Structure·D F MosherA Vaheri
Jan 1, 1978·The Anatomical Record·A C EndersS Schlafke
Apr 1, 1978·Developmental Biology·B W Mayer, D S Packard
Apr 1, 1978·The Journal of Experimental Medicine·S Stenman, A Vaheri
Sep 1, 1978·The Journal of Cell Biology·E CrouchP Bornstein
Apr 30, 1976·Biochimica Et Biophysica Acta·R O Hynes
Aug 1, 1976·American Journal of Obstetrics and Gynecology·A B ChenG I Solish
Jun 4, 1976·Differentiation; Research in Biological Diversity·J WartiovaaraA Vaheri
Jul 1, 1975·The Journal of Experimental Medicine·E LinderJ Wartiovaara
Jun 1, 1975·Developmental Biology·R M PrattM C Johnston
Oct 18, 1973·Biochimica Et Biophysica Acta·E RuoslahtiE Linder
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R O Hynes
Aug 1, 1974·The Journal of Cell Biology·R O Hynes, K C Humphryes
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K M Yamada, J A Weston
Aug 1, 1970·The Journal of Experimental Zoology·F J Manasek
Aug 1, 1980·The Anatomical Record·R E Waterman, G C Schoenwolf

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Citations

Jun 1, 1996·The Journal of Experimental Zoology·W C HamlettE Cuevas
Jan 1, 1985·Anatomy and Embryology·J M Icardo
Jul 1, 1989·The Histochemical Journal·D B Wilson, D P Wyatt
Jan 1, 1983·Developmental Biology·J M Icardo, F J Manasek
May 19, 2007·Seminars in Cell & Developmental Biology·A Dickinson, H Sive
Jul 19, 2012·Journal of Anatomy·Vladimír SoukupRobert Cerny
May 9, 2006·Developmental Biology·Amanda J G Dickinson, Hazel Sive
Jul 12, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lucia ManniPaolo Burighel
Apr 1, 1985·The Anatomical Record·R E Waterman
Feb 1, 1993·The Anatomical Record·M C DeRuiterA C Gittenberger-De Groot
Apr 1, 1987·The Anatomical Record·G T KittenD L Bolender
Jun 1, 1986·Journal of Cellular Physiology·E J WaterhouseG Balian
May 1, 1983·The Journal of Investigative Dermatology·D L NelsonG Balian

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