PMID: 6402419Feb 1, 1983Paper

Indirect immunoperoxidase localization of aflatoxin B1 in rat liver

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
J J PestkaF S Chu

Abstract

Aflatoxin B2a (AFB2a) antibody was used as a histochemical probe in the indirect immunoperoxidase localization of aflatoxin B1 (AFB1) bound to rat liver. The efficacy of the indirect method was initially demonstrated by detecting AFB1 covalently bound to DNA in an enzyme-linked immunosorbent assay. AFB1-modified DNA was attached to a polystyrene microtissue culture plate (solid phase) and then subjected to sequential incubation with AFB2a antiserum followed by goat anti-rabbit peroxidase conjugate. Assays for bound peroxidase revealed that the AFB2a antiserum could be diluted 200,000-fold and still yield a signal-to-noise ratio of 10 when compared to an unmodified DNA control. When the same indirect immunoperoxidase protocol was applied to the light-microscopic localization of AFB1 in liver sections of rats treated in vivo with the mycotoxin, bound toxin could be identified in excellent detail in tissues fixed with periodate-lysine-paraformaldehyde and embedded in glycol methacrylate, but was detectable with only poor resolution in unfixed cryostat sections. Peroxidase-positive reactions in hepatocytes typically exhibited strong nuclear and relatively lighter cytoplasmic staining. Greater concentrations of peroxidase-positive h...Continue Reading

References

May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J M EssigmannG N Wogan
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G de MurciaM Leng
Jan 1, 1968·Histochemie. Histochemistry. Histochimie·I Okrös
Nov 1, 1974·Sabouraudia·D B LouriaM Buse
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky
Jun 1, 1981·The Journal of Pathology·W H Butler, V Hempsall
Oct 30, 1951·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·M ABERCROMBIE, R D HARKNESS

❮ Previous
Next ❯

Citations

Jan 1, 1991·Critical Reviews in Food Science and Nutrition·U SamarajeewaM R Marshall
Jul 1, 2010·Prikladnaia biokhimiia i mikrobiologiia·A E UrusovB B Dzantiev
Feb 1, 1984·Toxicology and Applied Pharmacology·S C LeeF S Chu
Aug 5, 2010·Mutation Research·José Manuel Pérez MartínMaría José Hazen
Sep 16, 1991·Biochemical and Biophysical Research Communications·J I EwaskiewiczJ J Ch'ih
Jan 1, 1987·Die Nahrung·T Müller
Mar 1, 1984·Applied and Environmental Microbiology·J J Pestka, F S Chu
Jul 18, 1984·Biochemical and Biophysical Research Communications·J J Ch'ih, T M Devlin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.