Indirect laser-induced fluorescence detection for capillary electrophoresis using a violet diode laser

Analytical Chemistry
Jeremy E MelansonCharles A Lucy

Abstract

The violet (415 nm) diode laser is used for indirect laser-induced fluorescence detection in capillary electrophoretic separations of inorganic anions and chemical warfare agent degradation products. Inorganic anions were detected using 8-hydroxypyrene-1,3,6-trisulfonic acid as the indirect probe and achieved submicromolar (40-80 ppb) detection limits in a 2-min separation. The chemical warfare agent degradation products methylphosphonic acid, ethyl methylphosphonate, isopropyl methylphosphonate, and pinacolyl methylphosphonate were detected using the porphyrin tetrakis(4-sulfophenyl)porphine as the indirect probe and achieved detection limits of 0.1 microM (9 ppb), which are 1 order of magnitude better than that achieved using indirect UV detection. Baseline stability achieved with the violet diode laser was excellent, with dynamic reserve (DR) values of > 1000, which are 15 times better than that achieved using an unstabilized HeCd laser.

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Citations

Sep 28, 2002·Journal of Chromatography. a·T de BeerC Gooijer
Dec 20, 2002·Journal of Chromatography. a·Edwin W J HooijschuurUdo A Th Brinkman
Jan 22, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·An-Kai Su, Cheng-Huang Lin
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Mar 4, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yen-Sheng ChangCheng-Huang Lin
Nov 10, 2014·Analytical and Bioanalytical Chemistry·Tjorben Nils PoschCarolin Huhn
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Sep 7, 2007·Electrophoresis·Pavel CoufalKarel Stulík
Aug 23, 2011·The Analyst·Svetlana S AleksenkoAndrei R Timerbaev
Jul 1, 2006·Analytical Chemistry·You-Ren ShuCheng-Huang Lin

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