Indirect oxidation of ferrocyanide by peroxynitrite--evidence against the formation of hydroxyl radicals

Nitric Oxide : Biology and Chemistry
S Goldstein, G Czapski

Abstract

Thermodynamic calculations by different assumptions for arriving to the standard entropy of the formation of peroxynitrite anion in water lead to opposite conclusions considering the feasibility of the homolysis of ONOOH to .OH and .NO2 (Koppenol et al. (1992). Chem. Res. Toxicol. 5, 834-832; Merenyi (1997). The First International Conference on the Chemistry and Biology of Peroxynitrite). Nevertheless, the formation of hydroxyl radicals is not guaranteed even if it is feasible on thermodynamic grounds. To test the role of hydroxyl radicals during the isomerization of ONOOH, the indirect oxidation of ferrocyanide by peroxynitrite was studied in the presence of formate ions and methanol, where all the relevant rate constants of the primary and secondary reactions involving hydroxyl radicals are known. The oxidation rates were unaffected by these scavengers, while the decrease in the oxidation yields was not correlated with the known rate constants of these scavengers with hydroxyl radicals. It is concluded that the indirect oxidation of ferrocyanide by peroxynitrite takes place through an unidentified intermediate (ONOOH*), which is not a cage of .OH and .NO2.

References

Jan 10, 1995·Archives of Biochemistry and Biophysics·L BrunelliJ S Beckman
Jan 1, 1996·Free Radical Biology & Medicine·S GoldsteinG Czapski

❮ Previous
Next ❯

Citations

Sep 26, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Willem H KoppenolHeinz Rüegger
Sep 23, 2014·Free Radical Biology & Medicine·Fernando Cruvinel DamascenoJosé Carlos Toledo
Jun 13, 2014·PloS One·Ramanna HemaMakarla Udayakumar
Apr 18, 2018·The Journal of Biological Chemistry·Fernando Cruvinel DamascenoJosé Carlos Toledo
Sep 19, 1998·Free Radical Biology & Medicine·G L Squadrito, W A Pryor

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.