Indirect regulation of TFPI-2 expression by miR-494 in breast cancer cells.

Scientific Reports
Marianne S AndresenGrethe Skretting

Abstract

TFPI-2 has been shown to be involved in breast cancer pathogenesis by inhibiting extracellular matrix degradation, and low levels are associated with disease progression. As microRNA-494 (miR-494) protects against breast cancer progression, we investigated whether miR-494 is involved in the regulation of TFPI-2 in MCF-7 breast cancer cells. TFPI-2 mRNA and protein levels increased after transfection with miR-494 mimic, and TFPI-2 mRNA and miR-494 levels correlated positively in tumors from breast cancer patients. No specific binding sites for miR-494 in the 3'-untranslated region (UTR) of TFPI2 were identified; however, miR-494 was predicted in silico to bind 3'-UTR of the transcription factors AHR and ELF-1, which have potential binding sites in the TFPI2 promoter. ELF-1 mRNA was downregulated whereas AHR mRNA levels were upregulated after transfection with miR-494 mimic. Knockdown of ELF-1 and AHR increased and reduced TFPI-2 mRNA levels, respectively. Increased luciferase activity was seen when TFPI-2 promoter constructs containing the potential AHR or ELF-1 binding sites were co-transfected with miR-494 mimic. In conclusion, TFPI-2 mRNA levels were upregulated by miR-494 in MCF-7 breast cancer cells most likely by an indire...Continue Reading

References

Feb 20, 1995·Archives of Biochemistry and Biophysics·C N RaoD T Woodley
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C A SprecherD C Foster
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·J V SchmidtC A Bradfield
Jan 28, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·M IinoW Kisiel
Nov 11, 1999·Oncogene·P L DavisJ R Marks
Jun 26, 2003·Nucleic Acids Research·Domènec FarréXavier Messeguer
Dec 14, 2007·Chemical Research in Toxicology·Linh P Nguyen, Christopher A Bradfield
Jan 11, 2008·Nature·Sohail F TavazoieJoan Massagué
Jan 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Robert F PlaceRajvir Dahiya
Aug 1, 2008·Nature·Matthias SelbachNikolaus Rajewsky
Jan 30, 2009·Journal of Cellular and Molecular Medicine·Yu Wang, Caroline G L Lee
Nov 28, 2009·Nature Reviews. Clinical Oncology·Britta Weigelt, Jorge S Reis-Filho
Mar 30, 2011·PloS One·Markus RingnérJohan Staaf
Nov 5, 2011·Nucleic Acids Research·Vera HuangLong-Cheng Li
Jul 12, 2013·Carcinogenesis·María Contador-TrocaPedro M Fernández-Salguero
Dec 4, 2013·International Journal of Oncology·Rupninder SandhuWilliam B Coleman
Mar 13, 2014·Journal of Gastroenterology and Hepatology·Weiling HeZunfu Ke
Jun 19, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·WenCheng ZhangGuangshun Wang
Jan 30, 2015·Biological Reviews of the Cambridge Philosophical Society·Jen N GohAlan P Kumar
Jan 20, 2016·Genetics and Molecular Research : GMR·Y B MaB M Shi
Jun 1, 2016·Frontiers in Cell and Developmental Biology·Sonia Mulero-Navarro, Pedro M Fernandez-Salguero
Aug 10, 2016·Thrombosis Research·Mari TinholtNina Iversen
Jan 6, 2017·Cell Death & Disease·Meng-Na ZhanQian Zhao
Jan 16, 2017·Molecular and Cellular Endocrinology·Marianne S AndresenGrethe Skretting
Dec 21, 2017·Pharmacology & Therapeutics·Ángel C RomanPedro M Fernández-Salguero
Feb 24, 2018·Molecular Cancer·Hongyu LiuYongguang Tao
Mar 1, 2018·Frontiers in Immunology·Ping XueYufeng Zhou
Mar 13, 2019·Journal of Cellular Biochemistry·Grethe SkrettingBenedicte Stavik

❮ Previous
Next ❯

Citations

Sep 7, 2020·Progrès en urologie : journal de l'Association française d'urologie et de la Société française d'urologie·J LiuX Yan
Jun 30, 2021·The Journal of Obstetrics and Gynaecology Research·Hiroshi Kobayashi, Shogo Imanaka
Jul 13, 2021·Frontiers in Oncology·Jing MoBaocun Sun
Jul 10, 2021·Proteomics. Clinical Applications·Richard D SembaAlka Mahale

❮ Previous
Next ❯

Datasets Mentioned

BETA
RC209832

Methods Mentioned

BETA
transfection
PCR
Assay

Software Mentioned

GraphPad Prism
TargetScan
miRSVR
PROMO

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Gastroenterology and Hepatology
Weiling HeZunfu Ke
European Review for Medical and Pharmacological Sciences
X-Q ZhaoJ-W Fu
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Jialing YuanMingrong Xi
© 2021 Meta ULC. All rights reserved