Indirect Sertoli cell-mediated ablation of germ cells in mice expressing the inhibin-alpha promoter/herpes simplex virus thymidine kinase transgene

Biology of Reproduction
Maarit AhtiainenIlpo Huhtaniemi

Abstract

In the present study, we describe a novel mouse model for inducible germ cell ablation. The mice express herpes simplex virus thymidine kinase (HSV-TK) under the inhibin-alpha subunit promoter (Inhalpha). When adult transgenic (TG) mice were treated with famciclovir (FCV) for 4 wk, their spermatogenesis was totally abolished, with only Sertoli cells and few spermatids remaining in the seminiferous tubules. However, testicular steroidogenesis was not affected. Shorter treatment periods allowed us to follow up the progression of germ cell death: After 3 days, spermatogonia and preleptotene spermatocytes were no longer present. After a 1-wk treatment, spermatogonia, preleptotene, and zygotene spermatocytes were missing and the amount of pachytene spermatocytes was decreased. After a 2-wk treatment, round and elongating spermatids were present. During the third week, round spermatids were lost and, finally, after a 4-wk treatment, only Sertoli cells and few spermatids were present. Interestingly, the transgene is detected in Leydig and Sertoli cells but not in spermatogonia. This suggests that FCV is phosphorylated in Sertoli cells, and thereafter, leaks to neighboring spermatogonia, apparently through cell-cell junctions present, ...Continue Reading

References

Aug 1, 1992·The Journal of Endocrinology·A MooreI D Morris
Apr 1, 1992·Baillière's Clinical Endocrinology and Metabolism·B Jégou
Jul 15, 1992·Biochemical and Biophysical Research Communications·J G Su, A J Hsueh
Mar 26, 1990·Molecular and Cellular Endocrinology·A J GrootenhuisF H de Jong
Jul 9, 1990·Molecular and Cellular Endocrinology·G Verhoeven, J Cailleau
Nov 1, 1990·The Journal of Clinical Endocrinology and Metabolism·D J HandelsmanA F Phippard
Jan 1, 1987·Journal of Steroid Biochemistry·J M SaezM A Rivarola
Jan 1, 1987·International Review of Cytology·D M de Kretser
Jun 1, 1993·Biology of Reproduction·L A CookerE Goldberg
Apr 1, 1993·Endocrinology·A M HaavistoI Huhtaniemi
May 31, 1996·Molecular and Cellular Endocrinology·K KananenI Huhtaniemi
Aug 26, 1998·International Journal of Experimental Pathology·D G de Rooij
Jun 11, 1999·Biochemical and Biophysical Research Communications·S BaiL He
Apr 24, 2001·International Journal of Radiation Oncology, Biology, Physics·C MauduitM Benahmed
Dec 11, 2002·Journal of Biochemistry·Hideki OginoDai Ayusawa

❮ Previous
Next ❯

Citations

Jun 18, 2019·Journal of Animal Science and Biotechnology·Filipp SavvulidiLudek Stadnik
Oct 9, 2015·Andrology·L B SmithD Rebourcet
Oct 9, 2013·Biochemical and Biophysical Research Communications·Yukiko TomiokaEtsuro Ono

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

SigmaStat
SPSS

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.