Feb 1, 1986

Indirect spectrophotometric methods for the determination of antibiotics with iodine or periodate, and metol and sulphanilamide

Talanta
C S SastryU Viplava Prasad

Abstract

Two spectrophotometric methods are described for the determination of antibiotics. In the first method, penicillins, cephalosporins, streptomycin and griseofulvin are estimated by oxidizing them under neutral or slightly acidic conditions, after alkaline hydrolysis, by means of a known and excessive quantity of iodine solution. The excess of iodine is determined at pH 3.0 with metol and sulphanilamide. In the second method, dihydrostreptomycin, framycetin and the acid hydrolysis product of chloramphenicol are determined by a method involving oxidation with sodium metaperiodate, masking the excess of periodate with sodium molybdate, and using metol and sulphanilamide at pH 3.0 to determine the iodate formed. In both methods the absorbance of the resulting p-N-methylbenzoquinonemonoimine sulphanilamide charge-transfer complex is measured at 520 nm.

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Mentioned in this Paper

Sulfanilamide
Iodine solution
Dihydrostreptomycin
Antibiotic throat preparations
Iodine, Homeopathic preparation
Antifungal Antibiotics, Topical
Iodine
Cephalosporins
Oxidation
Antibiotics, Gynecological

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