Indirubin inhibits cell proliferation, migration, invasion and angiogenesis in tumor-derived endothelial cells

OncoTargets and Therapy
Zhuohong LiShijie Li

Abstract

Hepatocellular carcinoma is one of the most predominant malignancies with high fatality rate and its incidence is rising at an alarming rate because of its resistance to radio- and chemotherapy. Indirubin is the major active anti-tumor ingredient of a traditional Chinese herbal medicine. The present study aimed to analyze the effects of indirubin on cell proliferation, migration, invasion, and angiogenesis of tumor-derived endothelial cells (Td-EC). Td-EC were derived from human umbilical vein endothelial cells (HUVEC) by treating HUVEC with the conditioned medium of human liver cancer cell line HepG2. Cell proliferation, migration, invasion, and angiogenesis were assessed by MTT, wound healing, in vitro cell invasion, and in vitro tube formation assay. Td-EC were successfully obtained from HUVEC cultured with 50% culture supernatant from serum-starved HepG2 cells. Indirubin significantly inhibited Td-EC proliferation in a dose- and time-dependent manner. Indirubin also inhibited Td-EC migration, invasion, and angiogenesis. However, indirubin's effects were weaker on HUVEC than Td-EC. Indirubin significantly inhibited Td-EC proliferation, migration, invasion, and angiogenesis.

Citations

Sep 12, 2020·Chemical Biology & Drug Design·Bin SunYuwei Wang
Dec 5, 2019·Scientific Reports·Yuka TanakaMasutaka Furue
Jan 30, 2021·Natural Product Reports·Chang-Liang YaoDe-An Guo
Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kailong WangHaiyang Yu
May 9, 2021·Journal of Natural Medicines·Takaaki ShimizuHidekazu Tanaka

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Methods Mentioned

BETA
PCR
scraping

Software Mentioned

SPSS
ImagePro Plus

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