PMID: 7542798Feb 1, 1995Paper

Individual lymphocyte subset composition determines cAMP response to isoproterenol in mononuclear cell preparations from peripheral blood

Scandinavian Journal of Clinical and Laboratory Investigation
J H KnudsenN J Christensen

Abstract

The aim of the present study was to examine to what extent isoproterenol-stimulated cAMP production in mononuclear cells, isolated from resting human subjects, was correlated with lymphocyte subset composition. Peripheral blood was collected from 14 healthy male subjects, who rested in the sitting position. Mononuclear cells were prepared by density centrifugation and subset composition was determined by two-colour flow cytometry. Cyclic AMP production was determined by a radioimmunoassay after inhibition of phosphodiesterase and stimulation with isoproterenol. NK-cells (CD3-CD56+) were positively correlated to total cAMP concentrations (basal cAMP plus increase in cAMP). Tcytotoxic cells (CD8+) were positively correlated to the relative increase in cAMP. Thelper cells (CD4+) were negatively correlated to total cAMP concentrations and B-lymphocytes (CD(19+20)+) were negatively correlated to the relative increase in cAMP. The results support the view that NK-cells and Tcytotoxic cells have higher beta 2-adrenoceptor densities and more receptors with high affinity than Thelper and B-cells. In conclusion, our study demonstrates that cAMP levels in blood mononuclear cells obtained from normal subjects show substantial variation. Th...Continue Reading

References

Dec 1, 1987·European Journal of Clinical Investigation·E TønnesenM M Brinkløv
Aug 1, 1983·European Journal of Clinical Investigation·E Paietta, J D Schwarzmeier
May 1, 1994·Scandinavian Journal of Clinical and Laboratory Investigation·J H KnudsenN J Christensen
Apr 1, 1994·Scandinavian Journal of Clinical and Laboratory Investigation·T JohansenN Foged

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Citations

Mar 12, 2010·Intensive Care Medicine·Cheng-Ming TsaoChin-Chen Wu
Nov 20, 2009·The International Journal of Neuroscience·Joseph Prandota
Jan 23, 2003·The Journal of Pharmacology and Experimental Therapeutics·Hideo Kohka TakahashiMasahiro Nishibori
Jun 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S RahmouniM Moutschen

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