PMID: 9174268Apr 11, 1997Paper

Individual variation of human plantar stratum corneum lipids, determined by automated multiple development of high-performance thin-layer chromatography plates

Journal of Chromatography. B, Biomedical Sciences and Applications
S Zellmer, J Lasch

Abstract

The stratum corneum lipids are unique in composition and have been used frequently as a model system of the skin's lipid barrier. Automated multiple development (AMD) of high-performance thin-layer chromatography plates in combination with a 25-step gradient, based on methanol, diethyl ether and n-hexane separated the six major human plantar stratum corneum lipids. Post-chromatographic staining of these lipids with a solution of MnCl2-H2SO4 at 130 degrees C or a solution of CuSO4-H3PO4 at 140 degrees C allowed visualization of the lipids and quantification. The MnCl2-H2SO4 solution stained saturated fatty acids less intensely. Therefore, the CuSO4-H3PO4 solution was used for quantification and we found, on average, 2.06% (w/w) cholesterol 3-sulphate, 20.16% (w/w) free fatty acids, 20.25% (w/w) ceramides, 43.53% (w/w) non-esterified sterols, 4.56% (w/w) triacylglycerols and 9.4% (w/w) sterolesters in the human plantar stratum corneum extracts. The concentration of phospholipids was less than 1% (w/w). In addition, the lipid composition of twenty different human plantar stratum corneum extracts was determined. Statistics revealed a correlation between the ratio of free fatty acids and non-esterified sterols (r=0.832, p<0.01, n=20...Continue Reading

References

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Citations

Sep 27, 2005·Analytical and Bioanalytical Chemistry·Hany FarwanahKlaus Raith
Apr 2, 2005·Archives of Dermatological Research·Hany FarwanahJohannes Wohlrab
Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Rong Tsao, Zeyuan Deng
Aug 29, 1998·Chemistry and Physics of Lipids·S ZellmerJ Lasch
Oct 31, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·S ZellmerJ Lasch
Oct 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hany FarwanahKlaus Raith
Mar 21, 2006·Journal of Separation Science·Loïc QuintonPierre Chaminade
Jan 5, 2002·Journal of Chromatography. a·S L Abidi
Feb 5, 2003·Journal of Chromatography. a·Minlian ZhangZheng Li
Mar 21, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hany FarwanahKonrad Sandhoff
Dec 21, 2010·Journal of Chromatography. a·Beate FuchsJürgen Schiller

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