Individualized Transcriptional Resolution of Complicated Malaria in a Colombian Study

Journal of Personalized Medicine
Monica L Rojas-PeñaGreg Gibson

Abstract

To evaluate whether recovery from complicated malaria follows a common trajectory in terms of immunological mechanism or, rather, is highly individualized for each patient, we performed longitudinal gene expression profiling of whole blood. RNA sequencing (RNAseq) was performed on blood samples obtained from eight patients on four consecutive days between hospital admission and discharge. Six patients were infected with Plasmodium falciparum, and two with Plasmodium vivax; one patient was a pregnant woman infected with P. falciparum, who was hospitalized for several weeks. The characterization of blood transcript modules (BTM) and blood informative transcripts (BIT) revealed that patients' responses showed little commonality, being dominated by the balance of gene activity relating to lymphocyte function, inflammation, and interferon responses specific to each patient. Only weak correlations with specific complicated malaria symptoms such as jaundice, thrombocytopenia, or anemia were observed. The differential expression of individual genes, including transcripts derived from the human leukocyte antigen (HLA) complex, generally reflected differences in the underlying immune processes. Although the results of this pilot study do...Continue Reading

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Citations

Nov 5, 2019·Immunological Reviews·Tuan M Tran, Peter D Crompton

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Methods Mentioned

BETA
RNAseq

Software Mentioned

HTSeq
Tophat2
ToppFun
edgeR
R
JMP Genomics
PVCA
ToppGene suite

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