PMID: 2120185Oct 1, 1990Paper

Indoleacetic acid operon of Pseudomonas syringae subsp. savastanoi: transcription analysis and promoter identification

Journal of Bacteriology
T GaffneyT Kosuge

Abstract

Expression of the indoleacetic acid (iaa) operon, which contributes to the virulence of the phytopathogenic bacterium Pseudomonas syringae subsp. savastanoi, was monitored by using broad-host-range lacZ reporter gene plasmids. A combination of translational (gene) fusions and transcriptional (operon) fusions of P. syringae subsp. savastanoi sequences to lacZ allowed localization of the iaa operon promoter. RNA recovered from P. syringae subsp. savastanoi strains was mapped with iaa operon-specific probes to precisely locate the transcription initiation site. When transcripts from an iaaM::lacZ fusion in Escherichia coli were analyzed, an identical transcription initiation site was observed. The DNA sequence of the iaa operon promoter closely resembled the consensus E. coli promoter sequence. We detected an active, constitutive level of indoleacetic acid biosynthetic gene expression during bacterial growth under a variety of conditions in the absence of host plant influence.

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Citations

Dec 17, 2008·Protein Engineering, Design & Selection : PEDS·Pan-Fen WangMichael J McLeish
Jul 6, 2013·Biotechnology and Applied Biochemistry·Pramod W RamtekeBharat J Wadher
Jan 1, 1995·Critical Reviews in Microbiology·A Costacurta, J Vanderleyden
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Sep 18, 2012·Critical Reviews in Microbiology·Cheryl L PattenThomas J D Coulson
Feb 2, 2018·International Journal of Molecular Sciences·Mengsha LiJun Wu
Apr 25, 2003·The Journal of Biological Chemistry·Sejeong ShinByung-Ha Oh
Oct 22, 2009·Natural Product Reports·Harald Gross, Joyce E Loper
Jul 1, 1993·World Journal of Microbiology & Biotechnology·J L Ramos, S Marqués
Jan 11, 2020·Frontiers in Neuroscience·Harrisham KaurSharmila S Mande
Mar 20, 2020·Planta·Irina E DoduevaLudmila L Lutova
Mar 1, 1996·Canadian Journal of Microbiology·C L Patten, B R Glick
Sep 3, 2020·Applied Microbiology and Biotechnology·Daiana R Duca, Bernard R Glick

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