PMID: 6971096Sep 1, 1980Paper

Indoleamine 2,3-dioxygenase. A new, rapid, sensitive radiometric assay and its application to the study of the enzyme in rat tissues

The Biochemical Journal
J S CookS A Smith

Abstract

A simple and convenient assay for indoleamine 2,3-dioxygenase has been developed. This depends on the conversion of D-[ring-2-14C]tryptophan to [14C]formate, excess substrate is removed by adsorption onto charcoal. This assay, which is 20-fold more sensitive than previous procedures, is applicable both to crude extracts and to large numbers of samples. Activity in rat tissues is very much lower than in those of the rabbit; measureable activity is found only in the stomach, spleen, intestine and kidney. Enzyme activity in the rat intestine was increased by 50% in rats pretreated with L-tryptophan.

Citations

Aug 1, 1993·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·H Z Malina, X D Martin
Aug 2, 2013·Journal of Psychopharmacology·Abdulla A-B Badawy
Jan 23, 2014·Journal of Psychopharmacology·Abdulla A-B Badawy
Jan 23, 2014·Journal of Psychopharmacology·Simon N Young
Jan 30, 2004·Journal of Gastroenterology and Hepatology·Tetsuya IshioSeigo Kitano
Feb 12, 2004·Scandinavian Journal of Immunology·S KaiS Kitano
May 5, 2017·International Journal of Tryptophan Research : IJTR·Abdulla A-B Badawy
Jul 18, 2018·Metabolic Brain Disease·Yanjie QinYongning Lu
Nov 15, 2006·Cellular & Molecular Biology Letters·Derin B KeskinDebra A Gearhart
Sep 19, 2015·Bioscience Reports·Abdulla A-B Badawy
Jul 5, 2007·American Journal of Physiology. Renal Physiology·Kanishka MohibAnthony M Jevnikar
May 21, 2019·Egyptian Journal of Basic and Clinical Pharmacology·Abdulla Abu-Bakr Badawy
Nov 11, 2019·Experimental Gerontology·Abdulla A-B Badawy
Mar 7, 2021·International Journal of Molecular Sciences·Ruta ZulpaitePeter Schemmer
Nov 20, 2020·Pharmacology & Therapeutics·Ana DolšakMatej Sova
Aug 2, 2001·Biochimica Et Biophysica Acta·A BertazzoG Allegri
Jul 11, 1989·Biochimica Et Biophysica Acta·G Werner-FelmayerH Wachter
Apr 1, 1989·Archives of Biochemistry and Biophysics·J NaitoH Ogawa
Jun 30, 1986·Biochemical and Biophysical Research Communications·Y OzakiC A Nichol

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.