PMID: 9423859Jan 10, 1998Paper

Induced expression of the Legionella pneumophila gene encoding a 20-kilodalton protein during intracellular infection

Infection and Immunity
Yousef Abu Kwaik

Abstract

The eukaryotic protein synthesis inhibitor cycloheximid has been used by many investigators to selectively radiolabel intracellular bacteria. Although cycloheximide has no direct effect on bacterial gene expression, there are concerns that long-term inhibition of the host cell protein synthesis may have secondary effects on bacterial gene expression. Therefore, prior to further identification and cloning of the macrophage-induced (MI) genes of Legionella pneumophila, the effects of cycloheximide on L. pneumophila-infected U937 cells were evaluated by transmission electron microscopy. Inhibition of protein synthesis of the host cell for 6 h had no major effect on the ultrastructure of the host cell, on the formation of rough endoplasmic reticulum-surrounded replicative phagosome, or on initiation of intracellular bacterial replication. In contrast, by 15 h of cycloheximide treatment, there was profound deterioration in the host cell as well as in the phagosome. To examine protein synthesis by L. pneumophila during the intracellular infection, U937 macrophage-like cells were infected with L. pneumophila, and intracellular bacteria were radiolabeled during a 2-h cycloheximide treatment or following 12 h of cycloheximide treatment....Continue Reading

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Citations

Oct 1, 2010·Environmental Microbiology Reports·Souhaila Al-KhodorYousef Abu Kwaik
Mar 27, 1999·Molecular Microbiology·Y A Kwaik
Sep 24, 1999·Electrophoresis·Y A Kwaik, O S Harb
Jan 11, 2005·Applied and Environmental Microbiology·Maëlle MolmeretYousef Abu Kwaik
Aug 24, 1999·Infection and Immunity·L Y Gao, Y Abu Kwaik
Sep 3, 1998·Applied and Environmental Microbiology·Y Abu KwaikO S Harb
Dec 22, 1999·Microbes and Infection·O S Harb, Y Abu Kwaik
Jan 24, 2004·Microbes and Infection·Maëlle MolmeretYousef Abu Kwaik
Mar 4, 2006·Microbes and Infection·Helmy RachmanStefan H E Kaufmann
Dec 23, 2009·Journal of Proteomics·Marise Fonseca dos SantosGilberto Barbosa Domont
Apr 24, 2010·Veterinary Microbiology·Satoko KawajiRichard J Whittington

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