PMID: 8955670Oct 1, 1996Paper

Induced hypoxia in rat pulp and periapex demonstrated by 3H-misonidazole retention

Journal of Dental Research
K R BaumgardnerJ L Born

Abstract

Cellular hypoxia may be a useful indication of tissue distress in the dental pulp that could be used to investigate the early stages of pulpal responses. Tritiated misonidazole (3H-MISO) is a marker which preferentially labels cells with decreased oxygen tension (hypoxia). The experiments reported here were carried out to determine whether this agent could distinguish between hypoxic and normoxic pulp and periapical tissues. Rats were injected intra-peritoneally with either 3H-MISO, unlabeled MISO, or saline, then divided into normoxic, hypoxic, and control groups. Normoxic animals were maintained at ambient pressure. We induced hypoxia by maintaining animals in a hypobaric chamber at 0.5 atm for 24 hrs. 3H-MISO retention was assessed by quantitative analysis of tissue autoradiographs. 3H-MISO retention rates in normoxic animals showed little variation except for increased retention in mature ameloblasts and immature odontoblasts in the continually erupting incisor. In both incisor and molar pulps, hypobaric hypoxia significantly increased 3H-MISO retention when compared with normoxic controls. Hypobaric hypoxia also resulted in intense 3H-MISO retention in cellular cementum, periodontal ligament, osteocytes, and, occasionally,...Continue Reading

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Citations

Apr 21, 2010·European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry·S Alaluusua
Jun 13, 2001·Biochemical and Biophysical Research Communications·T CatronM K Walker
Jun 6, 2007·Australian Dental Journal·C Yu, P V Abbott
Jul 21, 2015·European Journal of Oral Sciences·Rivan SidalyJanne E Reseland
Sep 9, 2018·Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry·Darya DabiriSteven Harte
Sep 14, 1999·The American Journal of Physiology·J S DoddT S Gross
Jan 21, 2016·International Endodontic Journal·R A Alghaithy, A J E Qualtrough
May 18, 1999·The American Journal of Physiology·D M HallC V Gisolfi
Feb 18, 2019·Journal of Endodontics·Athina Maria MavridouPaul Lambrechts

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