PMID: 2486063Jan 1, 1989Paper

Induced interphase cell retraction: its reversal and EGF potentiation

Tissue & Cell
K H Sit, K P Wong

Abstract

Human epidermoid A431 carcinoma cells retract into globular shapes upon activation with epidermal growth factor (EGF) but not normal cells. We find that normal anchor-dependent cultured cells will retract from attachment on incubation in saline with pH upshifting. There is significant potentiation of that phenomenon by EGF prepulsing as seen in (a) dose- and time-response experiments quantitated by measuring profile area and perimeter of cells (b) 22Na+-uptake studies and (c) pHi measurements. EGF action by itself produces quantitatively significant retractions, although only to a very small degree. Blocking Na+/H+ exchanges using (a) trifluoperazine (150 microM), (b) amiloride (2 mM) and (c) Na+-omission could block the cell retractions. Retracted cells can be reversed in low [Na+]o during which rapid 22Na+-efflux is observed. A dose-response ouabain (1 mM) sensitive relationship exists between [Na+]o and retardation of the reversal phenomenon. Ouabain however does not prevent reversal in low [Na+]o, nor even in combination with high [Na+]o given the presence of Ca2+. Retraction into spherical shapes would maximize volume-holding for a given surface area, however they are quantitatively more bulgy than cells rounded by trypsin...Continue Reading

References

Nov 25, 1987·Biochimica Et Biophysica Acta·K TryggvasonT Salo
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S GrinsteinE W Gelfand
Jan 1, 1985·Annual Review of Physiology·K D Philipson
Nov 1, 1962·The Journal of Cell Biology·A C TAYLOR
Jan 9, 1914·Science·C M Child

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Citations

May 1, 1993·In Vitro Cellular & Developmental Biology. Animal·K H SitK P Wong
Apr 17, 1991·Biochimica Et Biophysica Acta·K H Sit, K P Wong
Feb 1, 1993·The Anatomical Record·K H SitK P Wong

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