PMID: 8600348Feb 1, 1996Paper

Induced mitotic death of HeLa cells by abnormal expression of c-H-ras

Mutation Research
E I MirandaA García-Carrancá

Abstract

When HeLa cells were selected for stable expression of a neo gene, linked either to mutated or wt c-H-ras genes, morphological examination of selected clones from several experiments revealed formation of giant multinucleated cells. These morphological alterations culminate in cell death, as a consequence of mitotic catastrophe (or mitotic death). Although clones expressing the mutated gene produced significantly larger numbers of these giant cells, those transfected with the normal allele were also found to produce significantly more giant multinucleated cells than non-transfected HeLa cells. Northern blot analysis of mRNA revealed overexpression of the normal H-ras gene in these clones. Chromatin structure analysis of these clones showed gross alterations, including the presence of micronuclei and heteroploid nuclei. Interestingly, odd numbers of nuclei were found in colonies of these giant cells. In addition, alterations in cell cycle parameters were observed, including the appearance of a subpopulation of cells with an abnormal content of DNA, probably representing dying cells. Our data support the notion that abnormal expression of H-ras contributes to mitotic catastrophe and death of a subpopulation of HeLa cells.

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Citations

Jun 14, 2005·Archives of Medical Research·Emilio Córdova-AlarcónEfraín Garrido
Oct 16, 2002·Mutation Research·Carla SantanaAlejandro García-Carrancá
May 26, 2007·Radiation Research·Yoshiko Asaka-AmanoTakayuki Kuriyama
Nov 13, 2010·Folia Histochemica Et Cytobiologica·D GrzankaA Grzanka
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Jean H Overmeyer, William A Maltese
Mar 10, 2010·Biochemical Pharmacology·Sreenath V Sharma, Jeff Settleman
Dec 31, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Cindrilla ChumduriPeter T Daniel
Aug 15, 2000·Leukemia·M V Blagosklonny
Jul 21, 2005·In Vitro Cellular & Developmental Biology. Animal·Maria Luiza S MelloB C Vidal
Aug 29, 2021·Viruses·Yakelin Díaz-TejedaAlejandro García-Carrancá
Dec 15, 2007·Genes & Development·Sreenath V Sharma, Jeffrey Settleman

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