Induced Natural Transformation of Acinetobacter calcoaceticus in Soil Microcosms.

Applied and Environmental Microbiology
Kaare Magne NielsenJ D van Elsas

Abstract

Factors affecting natural transformation of Acinetobacter calcoaceticus BD413 with homologous chromosomal DNA in a silt loam soil microcosm were investigated. Inducible transformation of declining populations of noncompetent A. calcoaceticus cells was detectable for up to 6 days when a simple carbon source, salts, and freshly added DNA were used. In two different experimental setups, the residence time in soil of induced cells could be increased to either 11 or 24 h before DNA addition without reduced transformation frequency; 200-to 1,000-fold fewer transformants were observed following the addition of water. These observations suggest that A. calcoaceticus remains transformable for several hours after its activation by nutrients in soil. Increasing the levels of phosphate salts significantly enhanced the numbers of transformants without increasing the recipient counts correspondingly. Variable levels of ammonium or divalent cations (Mg(sup2+) and Ca(sup2+)) did not have a similar major influence. Soil moisture content significantly affected the transformation frequency of A. calcoaceticus cells, with a general tendency of higher frequencies in drier soil. A minimal frequency was observed at around 35% soil moisture. The data ...Continue Reading

References

Nov 9, 1978·Molecular & General Genetics : MGG·J B Graham, C A Istock
Jan 3, 1991·Nature·J M SmithB G Spratt
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R N Reusch, H L Sadoff
Jan 1, 1983·Journal of Bacteriology·C A CarlsonJ L Ingraham
Sep 1, 1994·Microbiological Reviews·M G Lorenz, W Wackernagel
Feb 1, 1993·Journal of General Microbiology·R PalmenK J Hellingwerf
Aug 1, 1996·Applied and Environmental Microbiology·H G WilliamsG J Stewart
Oct 1, 1996·Applied and Environmental Microbiology·B BaurB Jenni
May 1, 1997·Applied and Environmental Microbiology·K M NielsenJ D van Elsas
May 1, 1991·Applied and Environmental Microbiology·J H PaulJ M Thurmond
Feb 1, 1994·Applied and Environmental Microbiology·A V OgramC A Pettigrew

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Citations

May 26, 2001·Applied and Environmental Microbiology·S DemanècheP Simonet
Oct 15, 2014·Genome Biology and Evolution·Marie TouchonEduardo P C Rocha
Oct 27, 2007·Environmental Biosafety Research·Deborah J SimpsonMartin J Day
Sep 3, 2005·Nature Reviews. Microbiology·Christopher M Thomas, Kaare M Nielsen
Oct 27, 2007·Environmental Biosafety Research·Jessica L RayMaureen O'Callaghan
Oct 27, 2007·Environmental Biosafety Research·Babette Richter, Kornelia Smalla
Jan 15, 2013·Applied and Environmental Microbiology·Stephanie H KungRodrigo P P Almeida
Jun 9, 2016·Antimicrobial Agents and Chemotherapy·German Matias TragliaMaria Soledad Ramirez
Oct 27, 2007·Environmental Biosafety Research·Kaare M NielsenDaniele Daffonchio
May 8, 2015·Journal of Medical Microbiology·Indranil Biswas
Mar 7, 2003·Applied and Environmental Microbiology·Larissa HendrickxStefan Wuertz
Feb 11, 2014·Nature Reviews. Microbiology·Calum JohnstonJean-Pierre Claverys
Jan 15, 2018·Current Microbiology·Brettni QuinnMaria Soledad Ramirez
Apr 18, 1998·Applied and Environmental Microbiology·F Gebhard, K Smalla
Jul 12, 2001·Research in Microbiology·V C Nwosu
Dec 18, 2013·Environmental Science & Technology·Daqing MaoP J J Alvarez

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