Induced plasma expander-like properties as a function of PEG-chains on extension arm facilitated PEGylation of albumin: "mushroom to brush-like" conformational transition of the PEG-albumin conjugate

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
Ranjit K SahuSeetharama A Acharya

Abstract

Plasma expander-like properties of albumin induced on hexa as well as dodecacPEGylation using Extension Arm Facilitated PEGylation platform make it an excellent resuscitation fluid. PEGylation induced changes in the structure, drug binding, and plasma expander-like properties of bovine serum albumin has been now investigated as a function of PEGylation. The molecular volume of albumin increases on PEGylation nearly linearly; in the beginning up to about six PEG chains are conjugated, then plateau off, while the viscosity and colloidal osmotic pressure change very little initially and then increase exponentially as a function of PEG chains conjugated. PEGylation has essentially no influence on the secondary structure or drug properties of albumin. Tryphtophyl fluorescence of albumin is quenched on PEGylation as a direct correlate of the changes in molecular radius of PEG-albumin. It is concluded that hexaPEGylated and dodecaPEGylated albumin belong to two different configurational states of PEG-albumin in terms of packing of PEG-chains on the molecular surface of the protein. The results suggest a transition of PEGylated albumin from the initial mushroom-like conformation to brush conformation as the PEGylation increases. The th...Continue Reading

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Citations

Aug 8, 2012·The American Journal of Emergency Medicine·Surapong ChatpunPedro Cabrales
Jun 1, 2011·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·K AnandaSeetharama A Acharya
May 31, 2016·Chembiochem : a European Journal of Chemical Biology·David PaleschTanja Weil
Sep 23, 2010·Pharmaceutical Development and Technology·Robert W PayneMark Cornell Manning
Nov 14, 2019·Physical Chemistry Chemical Physics : PCCP·Aravinda MunasingheCoray M Colina
Apr 3, 2019·The Journal of Physical Chemistry. B·Aravinda MunasingheCoray M Colina

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