Induced Pluripotent Stem Cells With Six Reprogramming Factors From Prairie Vole, Which Is an Animal Model for Social Behaviors

Cell Transplantation
Masafumi KatayamaTomokazu Fukuda

Abstract

Prairie voles show strong pair bonding with their mating partners, and they demonstrate parental behavior toward their infants, indicating that the prairie vole is a unique animal model for analysis of molecular mechanisms of social behavior. Until a recent study, the signaling pathway of oxytocin was thought to be critical for the social behavior of prairie voles, but neuron-specific functional research may be necessary to identify the molecular mechanisms of social behavior. Prairie vole pluripotent stem cells of high quality are essential to elucidate the molecular mechanisms of social behaviors. Generation of high-quality induced pluripotent stem cells (iPSCs) would help to establish a genetically modified prairie vole, including knockout and knock-in models, based on the pluripotency of iPSCs. Thus, we attempted to establish high-quality prairie vole-derived iPSCs (pv-iPSCs) in this study. We constructed a polycistronic reprogramming vector, which included six reprograming factors (Oct3/4, Sox2, Klf4, c-myc, Lin28, and Nanog). Furthermore, we evaluated the effect of six reprogramming factors, which included Oct3/4 with the transactivation domain (TAD) of MyoD. Implantation of the pv-iPSCs into immunodeficient mice caused a...Continue Reading

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Citations

Nov 13, 2018·Journal of Cellular Physiology·Masafumi KatayamaTomokazu Fukuda
Aug 28, 2020·World Journal of Stem Cells·Dharmendra KumarWilfried A Kues
Sep 17, 2019·World Journal of Stem Cells·Laís Vicari de Figueiredo PessôaKristine Karla Freude
Apr 20, 2021·Molecular Therapy. Methods & Clinical Development·Yoshihiro IwamotoShin Kaneko

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Methods Mentioned

BETA
transgenic
genetic modification
PCR
electrophoresis
Fluorescence
transfection

Software Mentioned

Multiplate RQ

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