Induced somatic sector analysis of cellulose synthase (CesA) promoter regions in woody stem tissues

Planta
Nicky M CreuxAntanas V Spokevicius

Abstract

The increasing focus on plantation forestry as a renewable source of cellulosic biomass has emphasized the need for tools to study the unique biology of woody genera such as Eucalyptus, Populus and Pinus. The domestication of these woody crops is hampered by long generation times, and breeders are now looking to molecular approaches such as marker-assisted breeding and genetic modification to accelerate tree improvement. Much of what is known about genes involved in the growth and development of plants has come from studies of herbaceous models such as Arabidopsis and rice. However, transferring this information to woody plants often proves difficult, especially for genes expressed in woody stems. Here we report the use of induced somatic sector analysis (ISSA) for characterization of promoter expression patterns directly in the stems of Populus and Eucalyptus trees. As a case study, we used previously characterized primary and secondary cell wall-related cellulose synthase (CesA) promoters cloned from Eucalyptus grandis. We show that ISSA can be used to elucidate the phloem and xylem expression patterns of the CesA genes in Eucalyptus and Populus stems and also show that the staining patterns differ in Eucalyptus and Populus s...Continue Reading

References

Feb 21, 1998·Science·T ArioliR E Williamson
Oct 12, 2000·Plant Physiology·T A Richmond, C R Somerville
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·W R ScheibleC Somerville
Dec 18, 2001·Plant Physiology·C PlomionA Stokes
Dec 14, 2002·Plant Physiology·Tom BeeckmanThomas Berleth
Jan 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Neil G TaylorSimon R Turner
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
Aug 24, 2004·Plant Cell Reports·Antanas V SpokeviciusGerd Bossinger
Jul 4, 2007·The Plant Journal : for Cell and Molecular Biology·Antanas V SpokeviciusGerd Bossinger
Feb 27, 2008·The New Phytologist·Neil G Taylor
Feb 3, 2009·Current Opinion in Plant Biology·Dario GrattapagliaRonald R Sederoff
Apr 21, 2009·Trends in Plant Science·Manoj KumarBrian E Ellis
Jun 2, 2009·Current Opinion in Biotechnology·Shawn D Mansfield
Aug 4, 2009·BMC Plant Biology·Yanbin YinYing Xu
Nov 7, 2009·PLoS Genetics·Margaret C W HoRobert A Drewell
Jun 16, 2010·The New Phytologist·Dongliang SongLaigeng Li
Sep 25, 2010·Trends in Genetics : TIG·Robin D Dowell
Mar 1, 2011·Annual Review of Plant Biology·Zoë A PopperDagmar B Stengel
Mar 13, 2012·Plant Biotechnology Journal·Jae-Heung KoKyung-Hwan Han
Apr 5, 2012·The New Phytologist·Eshchar MizrachiAlexander A Myburg

❮ Previous
Next ❯

Citations

Sep 28, 2014·Tree Physiology·Sanushka NaidooAlexander A Myburg
Apr 29, 2015·The Plant Journal : for Cell and Molecular Biology·Fabien Baldacci-CrespMarie Baucher
Oct 9, 2016·Plant Science : an International Journal of Experimental Plant Biology·Christoffer Johnsson, Urs Fischer
Dec 15, 2016·Journal of Experimental Botany·Rishikesh P Bhalerao, Urs Fischer
Oct 20, 2018·Frontiers in Plant Science·Michael NagleSteven H Strauss
Jan 9, 2021·Frontiers in Plant Science·Minghao MaFansuo Zeng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.